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注射毒品者中常见肝炎病毒感染,作者分别用聚合酶链反应(PCR)和酶免疫法(EIA)对含有乙型肝炎病毒(HBV)DNA和丙型肝炎病毒(HCV)RNA的注射器及含有抗HBV核心抗体和抗-HCV的注射器进行测定,并模拟注射器的使用以确定这些方法的敏感性.结果表明,PCR的平均敏感性:测HBV为0.082μl血液,保存时间对其敏感性无影响;测HCV为0.185μl血液,保存时间大于8个月,敏感性下降9倍,而对18SrRNA的敏感性无影响.EIA法的平均敏感性:测HBv为0.185μl血液,保存时间大于8个月,敏感性下降5倍;测HCV为0.023μl血
Common hepatitis virus infection among injecting drug users, the authors were using polymerase chain reaction (PCR) and enzyme immunoassay (EIA) for hepatitis B virus (HBV) DNA and hepatitis C virus (HCV) RNA syringes and containing anti HBV core antibody and anti-HCV syringes were measured and the use of syringes was simulated to determine the sensitivity of these methods.The results showed that the average sensitivity of PCR: HBV is measured as 0.082μl blood, the storage time has no effect on its sensitivity; Measured HCV was 0.185μl blood, the storage time was more than 8 months, the sensitivity decreased by 9 times, but had no effect on the sensitivity of 18SrRNA.The average sensitivity of EIA method: measured HBv was 0.185μl blood, the preservation time was more than 8 months, Sensitivity decreased by 5 times; measured HCV 0.023μl blood