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目的 :构建g3pN1 hCD2 0跨膜及胞外区基因表达载体 ,并在大肠杆菌中进行高效融合表达。方法 :利用反转录PCR和PCR方法 ,分别从Daudi细胞和M13K0 7噬菌体中扩增hCD2 0基因和编码g3pN1蛋白N1端的基因 ,并重组到pTIG Trx表达载体中 ,在大肠杆菌中融合表达。结果 :表达产物可被抗CD2 0分子的单克隆抗体 (mAb)识别。结论 :成功地表达并鉴定了目的蛋白。
Objective: To construct the g3pN1 hCD20 transmembrane and extracellular region gene expression vector and express it in E. coli. Methods: The hCD20 gene and the gene encoding the N1 end of g3pN1 protein were amplified from Daudi cells and M13K0 7 phage respectively by reverse transcription PCR and PCR, and were recombined into pTIG Trx expression vector and expressed in E. coli. Results: The expression product can be recognized by anti-CD20 molecule monoclonal antibody (mAb). Conclusion: The target protein was successfully expressed and identified.