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目的 观察川芎嗪对支气管哮喘 (简称哮喘 )大鼠模型气道重塑的抑制作用并探讨其作用机制。方法 32只SD大鼠按随机数字表法分成正常对照组 (A组 )、哮喘模型组 (B组 )、小剂量川芎嗪组 (C组 ,4 0mg/kg)和大剂量川芎嗪组 (D组 ,80mg/kg) ,以卵白蛋白 (OVA)致敏并长期吸入激发制备大鼠慢性哮喘模型。采用免疫组化半定量法测定气道壁胶原和转化生长因子 β1(TGF β1)含量 ,同时测定气道内、外径及平滑肌层、网状基底膜的厚度。结果 D组气道平滑肌层、网状基底膜的厚度为 (11 3± 1 3) μm、(11 3± 1 7) μm ,B组分别为 (19 7± 1 8) μm、(19 8± 1 6 ) μm ,两组比较差异有统计学意义 (P均 <0 0 1) ,但D组与A组 [(10 6± 1 2 ) μm、(9 8± 1 6 ) μm]、C组 [(11 6± 0 9) μm、(12 3± 1 8) μm]比较差异无统计学意义 (P均 >0 0 5 ) ;D组气道内外径比值为 0 77± 0 0 6 ,B组为0 6 3± 0 0 5 ,D组与B组比较差异有统计学意义 (P <0 0 1) ;D组气道壁Ⅲ型胶原及TGF β1含量吸光度 (A)值分别为 2 1± 5、2 6± 5 ,B组分别为 5 5± 7、6 9± 14 ,两组比较差异有统计学意义 (P <0 0 1) ,D组与C组 (32± 8、38± 10 )比较差异有统计学意义 (P <0 0 5 ) ,C组与B组比较差异也有统计学意义 (P <0 0
Objective To observe the inhibitory effect of tetramethylpyrazine on airway remodeling in asthmatic rat model and its mechanism of action. Methods Thirty-two SD rats were randomly divided into normal control group (group A), asthma model group (group B), low-dose ligustrazine group (group C, 40 mg/kg) and high-dose ligustrazine group (D). The rats (80 mg/kg) were sensitized with ovalbumin (OVA) and prolonged inhalation to prepare rat chronic asthma model. The immunohistochemical semi-quantitative method was used to measure the contents of collagen and transforming growth factor β1 (TGFβ1) in the airway wall. At the same time, the thickness of airway inner and outer diameter, smooth muscle layer and reticular basement membrane were measured. Results The thickness of airway smooth muscle and basal membrane in group D was (11 3± 1 3) μm and (11 3± 1 7) μm respectively. In group B, the thickness was (19 7± 1 8) μm and (19 8±). 16 μm, there was a statistically significant difference between the two groups (P <0 01), but D and A groups [(10 6 ± 1 2) μm, (9 8 ± 1 6) μm], Group C There was no significant difference between [(11 6 ± 0 9) μm, (12 3 ± 1 8) μm] (P all> 0 05), and the ratio of airway inside and outside diameter in group D was 0 77 ± 0 0 6. The group was 0 63 3 ± 0 05. The difference between group D and group B was statistically significant (P <0 01). The absorbance (A) value of type III collagen and TGF β 1 in the airway wall of group D was 2 1 ± 5, 2 6 ± 5, group B was 5 5 ± 7, 6 9 ± 14, the difference between the two groups was statistically significant (P <0 01), D group and C group (32 ± 8 and 38 ± 10) The difference was statistically significant (P < 0.05), and the difference between C and B was also statistically significant (P <0.