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目的 :了解抗癌药物顺铂 (CDDP)、阿霉素 (ADM)诱导肝细胞肝癌 (HCC)的细胞凋亡阈值。方法 :采用肿瘤细胞原代培养技术、活细胞萤光染色法和流式细胞仪定量分析。结果 :顺铂或阿霉素与细胞共同培养后 ,荧光显微镜下见正常细胞核呈弥散均匀荧光 ,凋亡细胞核内颗粒状荧光。随抗癌药物 (ADM、CDDP)剂量的增加 ,诱导人肝癌细胞的细胞凋亡率也增加 ,但以ADM 1.0 μg/mL和 2 0 μg/mL及CDDP 1 5 μg/mL和 3 0 μg/mL作用明显。ADM 2 0 μg/mL可导致HCC细胞凋亡 75 % ,CDDP 3 0 μg/mL可致HCC细胞凋亡 75 %。 2 5例未经治疗的HCC细胞和HepG - 2细胞的ADM、CDDP敏感性检测表明 ,ADM的凋亡阈值为 1 0 μg/mL ,CDDP的凋亡阈值为 1 5 μg/mL。临床凋亡敏感剂量分别为ADM 2 0mg/m2 ,CDDP 30mg/m2 。结论 :本文获得的ADM和CD DP诱导HCC细胞凋亡的临床阈值对临床肿瘤化疗有一定的指导意义
Objective : To understand the apoptosis threshold of anticancer drugs cisplatin (CDDP) and adriamycin (ADM)-induced hepatocellular carcinoma (HCC). Methods: Quantitative analysis of tumor cells using primary culture techniques, live cell fluorescence staining and flow cytometry. RESULTS: After cisplatin or adriamycin co-cultured with cells, the normal nuclei showed diffuse uniform fluorescence under fluorescent microscope, and the granular fluorescence in apoptotic nuclei. With increasing doses of anticancer drugs (ADM, CDDP), the apoptosis rate of induced human hepatoma cells also increased, but with ADM 1.0 μg/mL and 20 μg/mL and CDDP 1 5 μg/mL and 30 μg/mL. The effect of mL is obvious. ADM 20 μg/mL can lead to 75% apoptosis of HCC cells and CDDP 30 μg/mL can cause apoptosis of HCC cells by 75%. The sensitivity of ADM and CDDP detection in 25 untreated HCC cells and HepG-2 cells showed that the apoptosis threshold of ADM was 10 μg/mL and the apoptosis threshold of CDDP was 15 μg/mL. The sensitive doses for clinical apoptosis were ADM 20 mg/m2 and CDDP 30 mg/m2, respectively. Conclusion : The clinical thresholds of apoptosis induced by ADM and CD DP in HCC cells have certain guiding significance for clinical tumor chemotherapy.