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目的探讨纳米铁(Fe)、纳米氧化锌(ZnO)和纳米氧化镁(MgO)材料对小鼠血清乳酸脱氢酶(LDH)和α-羟丁酸脱氢酶(α-HBDH)活力的影响。方法分别以5g/Kg·wt剂量的纳米(Nano-)或微米(Micro-)粒径Fe、ZnO和MgO给小鼠一次经口灌胃,14d后处死取血分离血清分析LDH和α-HBDH活性变化。结果Nano-和Micro-Fe染毒小鼠血清LDH活力均比对照组明显降低(P<0.05),但Nano-Fe和Micro-Fe组小鼠血清LDH差异无显著性(P>0.05);对照、Nano-Fe和Micro-Fe三组小鼠血清α-HBDH差异无显著性(P>0.05)。Nano-MgO组小鼠血清LDH比对照组有所升高,但差异无显著性(P>0.05)。Micro-MgO组小鼠血清LDH比对照组和Nano-MgO组有明显升高(P<0.05);对照、Nano-MgO和Micro-MgO三组小鼠血清α-HBDH差异则无显著性(P>0.05)。两种粒径ZnO染毒组小鼠血清LDH和α-HBDH比对照组有显著性升高(P<0.05),其中Micro-ZnO组小鼠血清LDH和α-HBDH活力升高的更为显著(P>0.05)。结论Nano-Fe、Nano-ZnO和Nano-MgO材料对小鼠血清LDH及α-HBDH活性的影响与相应微米粒径材料有所不同。
Objective To investigate the effects of nano-iron (Fe), nano-ZnO and MgO on serum LDH and α-HBDH activity in mice . Methods Mice were orally gavaged with 5g / Kg · wt doses of nano-sized or micro-sized Fe, ZnO and MgO respectively. After 14 days, the mice were sacrificed and their blood serum was separated for analysis of LDH and α-HBDH Activity changes. Results Serum LDH activity in Nano- and Micro-Fe-treated mice was significantly lower than that in control group (P <0.05), but there was no significant difference in serum LDH between Nano-Fe and Micro-Fe mice (P> 0.05) There was no significant difference in serum α-HBDH between Nano-Fe and Micro-Fe mice (P> 0.05). Serum LDH in Nano-MgO group was higher than that in control group, but the difference was not significant (P> 0.05). The serum LDH of mice in Micro-MgO group was significantly higher than that in control group and Nano-MgO group (P <0.05). There was no significant difference in the serum levels of α-HBDH in mice of Nano-MgO and Micro-MgO > 0.05). The serum LDH and α-HBDH in the two groups of mice exposed to ZnO were significantly higher than those in the control group (P <0.05), and the activity of LDH and α-HBDH in the group of Micro-ZnO was significantly higher (P> 0.05). Conclusion The effect of Nano-Fe, Nano-ZnO and Nano-MgO on the serum LDH and α-HBDH activity in mice is different from that of the corresponding micron size materials.