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采用压片法对不同倍性蝴蝶兰品种小孢子母细胞减数分裂及成熟花粉进行观测,并利用流式细胞仪测定了花粉中的DNA含量,发现除了单倍性配子外,均有少数未减数配子发生。不同倍性蝴蝶兰品种中单倍性、二倍性、三倍性和四倍性花粉平均直径分别为11.4、17.2、19.1和20.3μm,花粉直径与倍性呈正相关关系(r=0.935,P<0.065);二倍体、三倍体和四倍体蝴蝶兰品种未减数雄配子的平均发生率分别为0.59%、2.39%和0.67%,三倍体品种发生未减数雄配子的频率明显高于二倍体和四倍体品种;二倍体、三倍体和四倍体品种均通过二分体和三分体的方式形成未减数雄配子,其中二分体的形成是由于小孢子母细胞不进行减数分裂Ⅰ,而正常进行减数分裂Ⅱ所致,三分体的形成可能是由于纺锤体异常定位形成三极纺锤体所致。
Using the tabletting method, we observed the meiosis and mature pollen of microspore mother cells of different ploidy Phalaenopsis species, and determined the content of DNA in the pollen by flow cytometry and found that except for the haploid gametes, Minor gametogenesis. The average diameter of haploid, diploid, triploid and tetraploid pollens in different ploidy Phalaenopsis species were 11.4, 17.2, 19.1 and 20.3 μm, respectively. The pollen diameter was positively correlated with ploidy (r = 0.935, P <0.065). The average incidences of non-reduced male gametes in diploid, triploid and tetraploid Phalaenopsis were 0.59%, 2.39% and 0.67%, respectively. The frequency of non-reduced male gametes in triploid varieties Were significantly higher than the diploid and tetraploid varieties; diploid, triploid and tetraploid varieties are formed by doubles and trisomy unintended male gametes, in which the formation of dyad is due to Microspore mother cells did not undergo meiosis Ⅰ, and normal meiosis Ⅱ due to the formation of triad may be due to the abnormal formation of spindle caused by the three-pole spindle.