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目的探讨链脲佐菌素(STZ)-糖尿病大鼠胃肠动力障碍和胃肠肌间神经丛胆碱能和氮能神经元的关系。方法45只 SD 大鼠随机分为对照组、糖尿病组和胰岛素组。成模后16周测定大鼠胃肠动力,观察胃肠肌间神经丛胆碱能和氮能神经元的形态变化。结果与对照组比较,糖尿病组大鼠胃肠动力减弱(P<0.01),胃窦、小肠肌间神经丛胆碱能神经元(6.6±2.9 vs 15.7±3.815.6±10.3 vs 22.6±7.4,P 均<0.01)和胃窦部氮能神经元(5.3±1.2 vs 11.8±2.2,P<0.01)计数显著降低。胰岛素组胃肠动力、胃窦部氮能神经元和小肠胆碱能神经元计数均显著高于糖尿病组(P 均<0.05),但均低于对照组(P 均<0.05)。结论 STZ-糖尿病大鼠胃肠动力障碍可能与胃肠肌间神经丛胆碱能和氮能神经损伤有关;胰岛素治疗能在一定程度上改善糖尿病胃肠动力障碍。
Objective To investigate the relationship between streptozotocin (STZ) -diabetic rat gastrointestinal motility disorder and cholinergic and nitrogen-capable neurons in gastrointestinal myenteric jungle. Methods 45 SD rats were randomly divided into control group, diabetes group and insulin group. After 16 weeks of operation, the gastrointestinal motility of rats was determined, and the changes of cholinergic and nitrogen-capable neurons were observed. Results Compared with the control group, the gastrointestinal motility of diabetic rats was weakened (P <0.01), and the number of cholinergic neurons in gastric antrum and small intestine was (6.6 ± 2.9 vs 15.7 ± 3.815.6 ± 10.3 vs 22.6 ± 7.4, P <0.01) and the number of NAC in gastric antrum (5.3 ± 1.2 vs 11.8 ± 2.2, P <0.01). Gastrointestinal motility, antral neuropeptide and small intestine cholinergic neurons counts in the insulin group were significantly higher than those in the diabetic group (all P <0.05), but lower than those in the control group (all P <0.05). Conclusion The gastrointestinal motility disorder in STZ-diabetic rats may be related to the cholinergic and nitric oxide damage of the gastrointestinal myenteric nerve. Insulin treatment can improve gastrointestinal motility disorders of diabetic patients to a certain extent.