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随老年人数增加,与年龄相关的器官功能减退的可能机制引起人们的关注。随年龄的增加,线粒体DNA(mt DNA)的损伤增多可能是此机制的主要因素。线粒体负责细胞的能量生成,mt DNA特点之一是比细胞核DNA更容易突变。且突变在人一生中积累。我们的实验显示:mt DNA发生缺乏随年龄增加而增加。如果mt DNA突变与老年性器官功能障碍有关,则①mt DNA突变到多少数少才出现临床症状与体征。因为一个线粒体有2～3个mt DNA拷贝,一个细胞中有几千个mt DNA分子;②mt DNA基因突变的机制是什么?我们用动力学PCR技术对
With the increase of the number of the elderly, the possible mechanism of age-related organ dysfunction has aroused people’s attention. As age increases, increased damage to mitochondrial DNA (mt DNA) may be a major factor in this mechanism. Mitochondria are responsible for the energy production of cells. One of the features of mt DNA is that it is easier to mutate than nuclear DNA. And mutations in the accumulation of human life. Our experiments show that lack of mt DNA increases with age. If mt DNA mutations associated with senile sexual organ dysfunction, ① mt DNA mutation to the number of rare cases of clinical symptoms and signs. Because a mitochondrion has 2-3 copies of the mt DNA, a cell has thousands of mt DNA molecules; mtDNA gene mutations in what is the mechanism? We use kinetic PCR technology to