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AIM To investigate the role of heat shock protein(HSP)-glycoprotein(gp)96 in dendritic cells(DCs)and lymphocytes induction in gastric cancer(GC). METHODS Human GC cell lines KATOIII,MKN-28 and SGC-7901were infected with adenovirus gp96 at a multiplicity of infection of 100.gp96-GC antigen peptide complexes were purified.MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)assay,lactate dehydrogenase(LDH)release assay and enzymelinked immunosorbent assay were used to determine allo-reactive T cell stimulation,natural killer(NK)cell activity and expression of cytokines(such as interleukin(IL)-10,IL-12,interferon(IFN)-γand tumor necrosis factor(TNF)-α),respectively.Effect of cytotoxic T lymphocyte(CTL)on DCs incubated with HSP-gp96was also evaluated by LDH release.All assays were performed in triplicate and the average values were reported.Comparison between groups was conducted using Student’s t test.RESULTS T cells incubated with HSP-gp96 exhibited a marked increase in proliferation in a dose-dependent manner(P<0.05).NK cell activity after gp96-GC peptide complex treatment was significantly higher than that after antigen peptide treatment(P<0.05).The activity of CTLs incubated with DCs from three GC cells lines was obviously higher than that stimulated by GC antigen at ratios of 50:1,25:1,10:1,and 5:1(P<0.05).Furthermore,the secretion of TNF-α,IL-10,IL-12(P70)and IFN-γmarkedly increased after incubation with HSP-gp96(P<0.05).CONCLUSION HSP-gp96 promotes T cell response,enhances DC antigen presentation and induces cytokine secretion,as well.HSP-gp96 has potential as immunotherapy for elimination of residual GC cells.
AIM To investigate the role of heat shock protein (HSP) -glycoprotein (gp) 96 in dendritic cells (DCs) and lymphocytes induction in gastric cancer (GC). METHODS Human GC cell lines KATOIII, MKN-28 and SGC-7901were infected with adenovirus gp96 at a multiplicity of infection of 100.gp96-GC antigen peptide complexes were purified. MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide assay, lactate dehydrogenase assay and enzymelinked immunosorbent assay were used to determine allo-reactive T cell stimulation, natural killer (NK) cell activity and expression of cytokines (such as interleukin (IL) -10, IL- 12, interferon (TNF) -α), respectively. Effect of cytotoxic T lymphocyte (CTL) on DCs incubated with HSP-gp96 was also evaluated by LDH release. All assays were performed in triplicate and the average values were reported. Comparison between groups was conducted using Student’s t test.RESULTS T cells incubated with HSP-gp96 exhibited a marked increase in p (P <0.05) .NK cell activity after gp96-GC peptide complex treatment was significantly higher than that after antigen peptide treatment (P <0.05). The activity of CTLs incubated with DCs from three GC cells lines was obviously higher than that stimulated by GC antigen at ratios of 50: 1, 25: 1, 10: 1 and 5: 1 (P <0.05) .Furthermore, the secretion of TNF-α, IL- (P70) and IFN-γmarkedly increased after incubation with HSP-gp96 (P <0.05) .CONCLUSION HSP-gp96 promotes T cell response, enhances DC antigen presentation and induces cytokine secretion, as well. HSP-gp96 has potential as immunotherapy for elimination of residual GC cells.