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The screening for Black rot(Xanthomonas campestris PV.campestris)resistance in broccoli (Brassica oleracea L.Var.italica Planch) lines plays an important role in breeding for Black rot resistance and is of great value to practical utilization. The broccoli in bred line“91-9318-4”was used as the experimental material in the trial.X 0-2 strain of Xanthomonas campestris PV.campestris was and purified from the infected broccoli plants in the field.The spraying method was adopted in the inoculation.The trial was designed as the randomized blocks of two factors with three replications.Factor A was the foliar age and factor B was the concetrations of inoculum.Factor A had three levels A 1,A 2 and A 3 (the 2nd 4th and 6th true leaf stage),factor B had three levels B 1,B 2 and B 3(10 6 counts/mL,10 7 counts/mL and 10 8 counts/mL). Results of this experiment showed that the best method for screening Black rot resistance in broccoli is ①foliar age:the 4th true leaf stage;②concetration of inoculums;10 7 counts/mL;③temperature at 20～30℃ (from the end of June to the end of July at he local climate condition.) 26 breeding materials of broccoli were identified for resistance to Black rot by means of the acquired method.One resistant breeding material was obtained,the identifying results showed that this screening method is practical.
The screening for Black rot (Xanthomonas campestris PV. Campestris) resistance in broccoli (Brassica oleracea L. Var .italica Planch) lines plays an important role in breeding for Black rot resistance and is of great value to practical utilization. The broccoli in bred line “91-9318-4” was used as the experimental material in the trial. X 0-2 strain of Xanthomonas campestris PV. Campestris was and purified from the infected broccoli plants in the field. The spraying method was adopted in the inoculation The trial was designed as the randomized blocks of two factors with three replications. Factor A was the foliar age and factor B was the concetrations of inoculum. Factor A had three levels A 1, A 2 and A 3 (the 2nd 4th and 6th true leaf stage), factor B had three levels B 1, B 2 and B 3 (10 6 counts / mL, 10 7 counts / mL and 10 8 counts / mL). The results of this experiment showed that the best method for screening Black rot resistance in broccoli is ①foliar age: the 4th true leaf stage; ② concetration of inoculums; 10 7 counts / mL; ③ temperature of 20-30 ° C. (from the end of June to the end of July at he local climate condition.) 26 breeding materials of broccoli were identified for resistance to black rot by means of the acquired method. One resistant breeding material was obtained, the identifying results showed that that screening method is practical.