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目的以缺氧/缺糖再给氧为模型,观察参麦注射液对神经细胞凋亡的抑制作用。方法流式细胞术检测凋亡细胞百分率,激光共聚焦扫描显微镜检测细胞内Ca2+浓度,荧光显微镜观察细胞形态学变化和坏死百分率。结果缺氧/缺糖5h后再给氧可诱导神经细胞凋亡和细胞坏死,并显著增加细胞内Ca2+浓度和LDH的释放。与对照组相比有显著性差异(P<0.01)参麦注射液能显著降低神经细胞凋亡及坏死的百分率,降低细胞内Ca2+浓度,减少LDH的释放,其作用随剂量增加而增加。结论参麦注射液具有拮抗海马神经细胞凋亡的作用,这种作用可能与其能降低细胞内Ca2+浓度有关。
Objective To observe the inhibitory effect of Shenmai injection on apoptosis of nerve cells by hypoxia/glucose reoxygenation model. Methods The percentage of apoptotic cells was detected by flow cytometry. The intracellular Ca2+ concentration was detected by confocal laser scanning microscopy. The morphological changes and percentage of necrosis were observed by fluorescence microscope. RESULTS: Hypoxia / hypoglycemia 5 h after reoxygenation induced neuronal apoptosis and necrosis, and significantly increased intracellular Ca 2+ concentration and LDH release. Compared with the control group, there was a significant difference (P<0.01). Shenmai injection can significantly reduce the percentage of apoptosis and necrosis of nerve cells, reduce intracellular Ca2+ concentration, and reduce the release of LDH, and its effect increases with increasing dose. Conclusion Shenmai injection can antagonize the apoptosis of hippocampal neurons, which may be related to the decrease of intracellular Ca2+ concentration.