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目的:探讨丹龙醒脑片对老龄大鼠脑缺血再灌注后NF-κB、ICAM-1表达的影响。方法:用大脑中动脉栓塞再通法建立脑缺血再灌注模型,将动物随机分为假手术组、模型组、丹龙醒脑片组、尼莫地平组,采用原位末端标记法(TUNEL)检测凋亡神经细胞,采用免疫组化法,观察NF-κB、ICAM-1蛋白的表达。结果:丹龙醒脑片能显著减轻神经细胞的损伤;假手术组有一定量的NF-κB、ICAM-1表达,缺血后NF-κB、ICAM-1迅速增加,丹龙醒脑片组能显著抑制NF-κB、ICAM-1的表达,模型组和丹龙醒脑片组比较差异有统计学意义(P<0.01,P<0.05)。结论:抑制由缺血再灌注损伤诱导的NF-κB、ICAM-1表达可能是丹龙醒脑片脑保护作用的机制之一。
Objective: To investigate the effect of Danlongxingnao Tablet on the expression of NF-κB and ICAM-1 in the aged rats after cerebral ischemia and reperfusion. Methods: The model of cerebral ischemia-reperfusion was established by middle cerebral artery occlusion reperfusion. The animals were randomly divided into sham-operated group, model group, danlongxingnao tablet group and nimodipine group. TUNEL ) Were used to detect apoptotic neurons. Immunohistochemistry was used to observe the expression of NF-κB and ICAM-1. Results: Dan Long Xingnao Tablet can significantly reduce the damage of nerve cells; a certain amount of NF-κB and ICAM-1 were expressed in sham-operated group, while NF-κB and ICAM-1 increased rapidly after ischemia, Significantly inhibited the expression of NF-κB and ICAM-1. There was significant difference between model group and Dan Long Xingnao Tablet group (P <0.01, P <0.05). CONCLUSION: Inhibition of NF-κB and ICAM-1 expression induced by ischemia-reperfusion injury may be one of the mechanisms of brain protection in DLSH.