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目的:以人胚胎神经干细胞和人胚肺成纤维细胞为对象,观察青蒿CQ-189对其体外增殖的影响,并对昆明小鼠主要脏器毒副作用和半数致死量进行观察,旨在探讨青蒿CQ-189的毒副作用。方法:采用MTT法检测青蒿CQ-189对人胚肺成纤维细胞增殖的影响,台盼兰拒染法检测人神经干细胞存活数量。采用小鼠尾静脉给药物法检测半数致死量,组织形态学观察其对小鼠主要器官的毒性作用。结果:青蒿CQ-189在对白血病细胞有较强的抑制作用的有效用药剂量范围内(3.125～12.5 mg.L-1)对人胚胎神经干细胞和人胚肺成纤维细胞的毒副作用较低,其对小鼠的半致死剂量为550 mg.kg-1,并且在较高浓度作用下对小鼠主要脏器无明显损伤。结论:青蒿CQ-189的毒副作用较低,是很有开发应用前景的抗肿瘤天然成分。
OBJECTIVE: To observe the effects of Artemisia annua CQ-189 on the proliferation of human embryonic neural stem cells and human embryonic lung fibroblasts in vitro and to observe the toxic and side effects and the median lethal dose of major organs in Kunming mice Side effects of Artemisia annua CQ-189. Methods: The effects of Artemisia annua L. CQ-189 on the proliferation of human embryonic lung fibroblasts were detected by MTT assay. The survival of human neural stem cells was detected by trypan blue exclusion. Mice were sacrificed by caudal vein to test the lethal dose, and histomorphology was used to observe the toxic effects on the major organs of mice. RESULTS: Artemisia annua L. CQ-189 had a lower toxicity to human embryonic neural stem cells and human embryo lung fibroblasts within the effective dosage range of 3.125 ~ 12.5 mg.L-1 for leukemic cells with strong inhibitory effect , Its semi-lethal dose to mice was 550 mg.kg-1, and there was no obvious damage to the major organs of mice under the action of higher concentration. CONCLUSION: Artemisia annua CQ-189 has low toxic and side effects and is an anti-tumor natural component with promising applications.