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目的揭示鸡血藤水提取物(A.E.)的抗coxsackievirus B3(CVB3)病毒的作用。方法采用BALB/c小鼠经腹腔注射感染Coxsackie B3病毒造成小鼠心肌炎模型,观察心指数、死亡率和病理切片。并通过RT-PCR比较鸡血藤水提取物在BALB/c小鼠体内抑制Coxsackie B3病毒的mRNA表达的效果。结果显示A.E.在小鼠体内具有明显的抗病毒作用。每日灌注A.E.能够明显降低病毒感染小鼠的死亡率、心指数和心脏病理切片坏死和浸润的程度。而且A.E.还能显著的降低CVB3病毒mRNA的表达。结论表明鸡血藤值得进一步研究以发现更多能够治疗肠道病毒感染的潜在成分。
Objective To reveal the anti-coxsackievirus B3 (CVB3) virus effect of A. spathis water extract (A.E.). Methods BALB / c mice were injected intraperitoneally with Coxsackie B3 virus to induce myocarditis in mice. Cardiac index, mortality and pathology were observed. The effect of aqueous extract of Jixiao Rattan on the mRNA expression of Coxsackie B3 virus in BALB / c mice was compared by RT-PCR. The results showed that A.E. had a significant antiviral effect in mice. Daily perfusion of A.E. significantly reduced the mortality, heart index, and extent of necrosis and infiltration of heart-disease sections in virus-infected mice. Moreover, A.E. can significantly reduce the expression of CVB3 virus mRNA. The conclusions suggest that M. sphaerothecae deserves further study to find out more potential to treat enteroviral infections.