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【目的】蜡蚧轮枝菌是一种防治植物病原物和害虫的生防菌,弄清其致病的分子基础具有重要的意义。【方法】利用Illumina HiS eq技术测序蜡蚧轮枝菌的cD NA文库,并进行RNA-Seq序列拼接和功能注释。【结果】共获得1634670条高品质reads,碱基GC含量48.50%。14856条Unigene经组装拼接后,在Nr、Swiss-Prot、COG和KEGG数据库中分别比对得到1467、9379、6518和4188条Unigenes。采用GO分类工具将21403条Unigenes分成24个功能组,主要包含在细胞组成(1369)、分子功能(1679)和生物学过程(1928)3个大类里,在COG数据库注释的基础上,把6518条Unigenes分成38个功能组,通过GO和COG丰度识别分类,聚类的主要是一般功能预测、次生代谢产物的合成、运输和分解代谢以及信号转导机制起作用的独立基因。能与KEGG路径匹配的108条Unigenes中大部分和新陈代谢途径及次生代谢产物的合成有关。【结论】这些结果将有助于找寻蜡蚧轮枝菌的致病因子,从而丰富其致病机理。
【Objective】 Verticillium lecanii is a biocontrol agent against plant pathogens and pests and it is of great importance to clarify the molecular basis of its pathogenesis. [Method] The cD NA library of Verticillium lecanii was sequenced by using Illumina HiS eq technique and the RNA-Seq sequence splicing and functional annotation were performed. 【Result】 A total of 1634670 high-quality reads were obtained, with a GC content of 48.50%. After 14856 Unigene were assembled and assembled, 1467, 9379, 6518 and 4188 Unigenes were obtained respectively in Nr, Swiss-Prot, COG and KEGG databases. 21403 Unigenes were divided into 24 functional groups using the GO classification tool. They were mainly included in the three categories of cell composition (1369), molecular function (1679) and biological process (1928). Based on the COG database annotation, The 6518 Unigenes are divided into 38 functional groups, identified by GO and COG abundance categories, and clustered primarily as independent genes for general function prediction, synthesis of secondary metabolites, transport and catabolism, and signal transduction mechanisms. The majority of the 108 Unigenes that match the KEGG pathway are involved in the synthesis of metabolic pathways and secondary metabolites. 【Conclusion】 These results will help to find out the pathogenic factors of Verticillium lecanii and enrich its pathogenesis.