伴血吸虫病的结肠癌和息肉中c-erbB-2表达及AgNOR的意义

• 作 者: 刘荣辉 刘蔚 刘勇 周淑明
• 机 构: 江西省南昌县血吸虫病防治站,江西省人民医院病理科
• 出 处: 新消化病学杂志
• 发表日期: 1997年08期
• 关键词: 结肠癌 AgNOR c-erbB-2 肠息肉 结肠肿瘤 结肠息肉／代谢 原癌基因蛋白质 免疫组织化学 腺管 癌基因

目的研究并存血吸虫病的结肠癌和肠息肉中ｃｅｒｂＢ２的表达，ＡｇＮＯＲ颗粒的数量及特点，探讨血吸虫病与结肠癌发生的关系．方法应用免疫组织化学ＬＳＡＢ法对３３例并存血吸虫病的结肠癌和肠息肉（结肠癌２１例，伴有肠系膜淋巴结转移１７例；肠息肉１２例）和２０例无血吸虫病的结肠癌和肠息肉（结肠癌１０例，伴有肠系膜淋巴结转移４例；肠息肉１０例，进行ｃｅｒｂＢ２标记，并作ＡｇＮＯＲ染色．结果肿瘤细胞中癌基因ｃｅｒｂＢ２的表达呈明显异质性．２１例并存血吸虫病的结肠癌中７例阳性（３３３％），其中５例伴有肠系膜淋巴结转移者均为强阳性；１０例无血吸虫病的肠癌中３例阳性（３００％），其中２例伴有肠系膜淋巴结转移者均为强阳性；１２例并存血吸虫病的肠息肉中５例阳性（４１７％）；１０例无血吸虫病的肠息肉中仅１例阳性（１００％）．并存血吸虫病的肠息肉中ｃｅｒｂＢ２的表达明显高于无血吸虫病的肠息肉（Ｐ＜００５）ＡｇＮＯＲ／核均数示２１例并存血吸虫病的肠癌为５８６±１７７；１０例无血吸虫病的肠癌为４７５±１１３；１２例并存血吸虫病的肠息内为３５２±１２２，其中５例ｃｅｒｂＢ２阳性的肠息肉为３９４±?

Objective To investigate the expression of cerbB2 in colon cancer and intestinal polyps of schistosomiasis and the number and characteristics of AgNOR particles in schistosomiasis and explore the relationship between schistosomiasis and the occurrence of colon cancer. Methods LSAB immunohistochemical method was used to investigate 33 cases of colon cancer and intestinal polyps coexisting with schistosomiasis (21 cases of colon cancer, 17 cases of mesenteric lymph node metastasis, 12 cases of intestinal polyps) and 20 cases of colorectal cancer and intestinal polyps without schistosomiasis. (10 cases with colon cancer, 4 cases with mesenteric lymph node metastasis, 10 cases with intestinal polyps, c erbB  2 markers, and AgNOR staining. The expression of oncogene c  erbB  2 in tumor cells was significantly heterogeneous Among the 21 coexisting cases of schistosomiasis, 7 cases were positive (33. 3%), among which 5 cases with mesenteric lymph node metastasis were strongly positive; 10 cases of schistosomiasis-free colorectal cancer were positive in 3 cases (300). %), of which 2 cases were associated with mesenteric lymph node metastasis were strongly positive; 12 cases of intestinal polyps with schistosomiasis were positive in 5 cases (41.7%); 10 cases of intestinal polyps without schistosomiasis was only 1 case of positive ( (100%). The expression of cerbB2 in intestinal polyps with schistosomiasis was significantly higher than that without schistosomiasis. Intestinal polyps (P<005)AgNOR/nucleus average showed 21 cases of coexisting schistosomiasis had 586±177; 10 cases of schistosomiasis had 475±113; Twenty-two cases of coexisting schistosomiasis had 3  52  1  22 in the intestinal tract, of which 5 cases of c  erbB  2 positive intestinal polyps were 3  94 ?.