论文部分内容阅读
[目的]研究饮水摄入六价铬对大鼠外周血DNA损伤和血浆氧化应激的影响。[方法]取雌、雄SD大鼠各40只随机分为4组,每组10只,每笼2只,4组分别自由摄入饮用水(对照)及30、100、300mg/L重铬酸钾(K2Cr2O7)水溶液4周,每周3次记录每笼饮水量并更换现配的K2Cr2O7溶液。实验结束时测定体重及心、肝、肾、肺、胃、脾组织重量,计算脏器系数和大鼠平均每日饮水量,并测定外周血DNA损伤及血浆中8-羟基脱氧鸟苷(8-hydroxydeoxyguanosine,8-OHdG)和丙二醛(malondialdehyde,MDA)的含量。[结果]雌鼠300mg/L组的体重增加量低于对照组(P<0.05);雌鼠300mg/L组、雄鼠100mg/L和300mg/L组的平均每日饮水量均低于对照组(P<0.05,P<0.01)。300mg/L组雌、雄大鼠DNA损伤均加重(P<0.05)。雌鼠100mg/L和300mg/L组血浆中8-OHdG含量及雌鼠3个剂量组和雄鼠100、300mg/L组血浆中MDA含量均显著高于对照组(P<0.05,P<0.01)。[结论]饮水暴露Cr(VI)可致大鼠外周血DNA损伤加重,并使血浆中8-OHdG和MDA的含量升高。
[Objective] To investigate the effect of drinking water hexavalent chromium on DNA damage and plasma oxidative stress in peripheral blood of rats. [Methods] Forty male and female SD rats were randomly divided into 4 groups with 10 rats in each group and 2 rats in each cage. Four groups were fed with drinking water (control) and 30,100 and 300 mg / L heavy chromium Potassium acid (K2Cr2O7) solution for 4 weeks, 3 times a week record the amount of water per cage and replace the existing K2Cr2O7 solution. The body weight, heart, liver, kidney, lung, stomach and spleen tissue weights were measured at the end of the experiment, and the organ coefficients of rats and the average daily drinking water were calculated. The DNA damage in peripheral blood and plasma 8-hydroxydeoxyguanosine -hydroxydeoxyguanosine, 8-OHdG) and malondialdehyde (MDA). [Results] The weight gain of 300mg / L group was lower than that of the control group (P <0.05). The average daily water intake of 300mg / L group, 100mg / L and 300mg / L group were lower than that of control group Group (P <0.05, P <0.01). The DNA damage in both 300 mg / L and 100 mg male rats was aggravated (P <0.05). The content of 8-OHdG in plasma of female rats at 100 mg / L and 300 mg / L and plasma concentrations of plasma at 3 and 3 levels in female and 100 and 300 mg / L groups were significantly higher than those in control group (P <0.05, P <0.01) . [Conclusion] Exposed to drinking water Cr (VI) can cause DNA damage in peripheral blood of rats and increase the content of 8-OHdG and MDA in the plasma.