论文部分内容阅读
目的了解新疆地区艾滋病(AIDS)病人真菌感染的菌种分布和优势菌株,并检测AIDS病人病原真菌对不同抗真菌药物的体外敏感性,为临床早期诊断及合理用药提供科学依据,并发现该地区的耐药情况。方法分别取AIDS病人口咽、呼吸道、消化道及泌尿、生殖道的标本,采用常规方法做真菌培养,用科玛嘉显色培养基进行鉴定,药敏试验采用ATBTMFUNGUS3试剂盒测定50株分离酵母菌对5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑、伏立康唑的体外敏感性。结果100株分离菌株中,念珠菌属为97株,其他菌属3株;其中白念珠菌85株,克柔念珠菌6株,热带念珠菌2株,光滑念珠菌1株。曲霉菌属2株,分别为烟曲霉和土曲霉;接合菌属1株,为根霉。结论新疆地区AIDS病人真菌感染仍以白念珠菌为优势菌种,并有罕见菌种出现。抗真菌药物以5-氟胞嘧啶、两性霉素B敏感性较高,而唑类存在不同程度的耐药。
Objective To understand the distribution and dominant strains of fungi in AIDS patients in Xinjiang and to detect the sensitivity of pathogenic fungi in AIDS patients to different antifungal agents in vitro so as to provide a scientific basis for early clinical diagnosis and rational drug use. The resistance situation. Methods The specimens of oropharynx, respiratory tract, digestive tract, urinary tract and reproductive tract were collected from AIDS patients. The fungi were cultured by the conventional method and identified by Kema Jia chromogenic medium. The drug susceptibility test was performed on 50 strains of yeast isolated using the ATBTM FUNGUS3 kit In vitro susceptibility of bacteria to 5-fluorocytosine, amphotericin B, fluconazole, itraconazole, voriconazole. Results Among the 100 isolates, Candida was 97 and other strains were 3 strains. Among them, Candida albicans 85, Candida krusei 6, Candida tropicalis 2 and Candida glabrata 1. Aspergillus 2 strains, respectively, Aspergillus fumigatus and Aspergillus terreus; a joint strain of genus Rhizopus. Conclusions Candida albicans is still the predominant species of fungal infection in AIDS patients in Xinjiang, and rare species appear. Antifungal agents to 5-fluorocytosine, amphotericin B higher sensitivity, while the azoles have varying degrees of resistance.