论文部分内容阅读
探讨洛伐他汀联合塞来昔布作用于宫颈癌细胞SiHa的关键分子及对下游信号分子的影响。分别用不同浓度的洛伐他汀和塞来昔布孵育宫颈癌细胞Si Ha,根据CCK-8试剂盒检测24 h的细胞增殖结果,计算两药的IC_(50)。用IC_(50)浓度的洛伐他汀和塞来昔布作用于SiHa,qPCR检测小窝蛋白-1的mRNA水平变化以及Akt、ERK1/2和STAT3的磷酸化水平。SiHa细胞经洛伐他汀和塞来昔布处理24 h后,IC_(50)值分别为25μmol/L和50μmol/L;洛伐他汀或塞来昔布单独处理Si Ha细胞24 h,均可从蛋白水平抑制小窝蛋白-1的表达;并且洛伐他汀可协同塞来昔布降低小窝蛋白-1引起的Akt、ERK1/2和STAT3信号分子磷酸化。洛伐他汀和塞来昔布联合应用下调宫颈癌细胞小窝蛋白-1的表达,并且可以显著性抑制下游信号分子的激活。
To investigate the effect of lovastatin combined with celecoxib on the key molecules of SiHa in cervical cancer cells and its influence on downstream signaling molecules. Cervical cancer cells Si Ha were incubated with different concentrations of lovastatin and celecoxib, and the IC 50 of the two drugs was calculated according to the results of cell proliferation detected by CCK-8 kit for 24 h. The effects of lovastatin and celecoxib with IC 50 concentration on SiHa were detected by qPCR. The levels of phosphorylated Akt, ERK1 / 2 and STAT3 were detected by qPCR. SiHa cells treated with lovastatin and celecoxib for 24 h, the IC 50 values were 25 μmol / L and 50 μmol / L, respectively. Lovastatin or celecoxib alone treated Si Ha cells for 24 h, Protein level inhibits caveolin-1 expression; and lovastatin may cooperate with celecoxib to reduce caveolin-1-induced phosphorylation of Akt, ERK1 / 2 and STAT3 signaling molecules. The combination of lovastatin and celecoxib down-regulated the expression of caveolin-1 in cervical cancer cells and significantly inhibited the activation of downstream signaling molecules.