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蛋白质表面相互作用对药物释放、生物传感器、亲和色谱和离子色谱以及仿生材料在生物环境中均起到关键作用。许多技术可用来研究不同材料界面间的蛋白质密度、结构和取向。全内反射技术由于具有快速、无损、灵敏以及灵活等特点,在研究分子水平蛋白质界面耦合过程发挥着重要的作用。描述了全内反射的原理、技术以及在检测和控制生物分子间作用等方面的应用,主要包括蛋白质—蛋白质,蛋白质-DNA,DNA-DNA以及蛋白质—膜等界面体系。这些研究有助于进一步加强对蛋白质表面相互作用的理解。近年来全内反射技术在蛋白质领域的应用,为其在生物物理和生物化学方面的应用提供了依据和可能。
Protein surface interactions play a key role in drug release, biosensors, affinity and ion chromatography, and biomimetic materials in the biological environment. Many techniques are available to study the density, structure and orientation of proteins at different material interfaces. Total internal reflection technology plays an important role in the study of protein-protein interface coupling at the molecular level because of its fast, non-destructive, sensitive and flexible properties. The principle of total internal reflection, its technology and its applications in the detection and control of the interaction between biological molecules are described. The main applications include interface proteins such as protein-protein, protein-DNA, DNA-DNA and protein-membrane. These studies help to further enhance understanding of protein surface interactions. In recent years, total internal reflection technology in the field of protein applications for its biophysical and biochemical applications provide the basis and possibility.