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目的:研究纳米硒的抗氧化及抗肿瘤活性。方法:以桔梗多糖为模板在温和的条件下制备纳米硒(Nano Se0)。采用邻苯三酚自氧化法测定纳米硒—桔梗多糖对超氧阴离子自由基的清除作用,水杨酸法测定羟自由基清除作用,紫外法测定DPPH自由基清除作用,MTT法研究其体外抗肿瘤活性。结果:纳米硒和桔梗多糖均显示出与浓度正相关的抗氧化活性,且纳米硒的抗氧化活性比桔梗多糖更强。纳米硒对Hep G-2细胞显示出更强的体外抗肿瘤活性,IC50值为123.7μg/ml。结论:纳米硒—桔梗多糖具有很强的抗氧化和抗肿瘤活性,为开发纳米硒—桔梗多糖的功能产品提供理论支撑。
Objective: To study the anti-oxidation and anti-tumor activity of nano-selenium. Methods: Nanometer selenium (Nano Se0) was prepared under placental conditions with platycodon polysaccharide as a template. The pyrogallol autoxidation method was used to determine the scavenging effect of Nano-Selenium-Platycodon polysaccharides on superoxide anion free radicals. Salicylic acid method was used to determine the scavenging effect of hydroxyl free radicals. DPPH radical scavenging activity was measured by ultraviolet spectrophotometry. Tumor activity. Results: The polysaccharides of Nano Selenium and Platycodon both showed antioxidant activity positively correlated with the concentration, and the antioxidant activity of Nano Selenium was stronger than that of Platycodon. Nanoselenium showed stronger in vitro anti-tumor activity on Hep G-2 cells with an IC50 value of 123.7 μg / ml. Conclusion: Nano-Selenium-Platycodon polysaccharide has strong anti-oxidation and anti-tumor activity, providing theoretical support for the development of functional products of Nano Selenium-Platycodon.