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为了解猫螺杆菌(Hf)和霍乱毒素共同免疫小鼠能否产生长期保护作用,作者分别在第1、3、6、30、54天用1mg超声处理的Hf菌体加10μg霍乱毒素口饲7～8周龄的SPF雄性BALB/c小鼠.分别于末次免疫后1、3、6、13、15个月用Hf活菌攻击小鼠,攻击后21天杀死动物,取其胃组织进行尿素酶试验和Giemsa染色,检测有无螺杆菌存在;同时用ELISA测血清IgG和唾液IgA抗Hf抗体.结果表明,在免疫后1、6、13、15个月时,所有免疫小鼠均能抵御Hf攻击,Hf清除率达100%,3个月时清除率为86%(尿素酶法)或93%(Giemsa法).与注射生理盐水的对照组相比,两组间的清除率有显著差异.免疫组和对照组的IgG抗体水平因个体免疫应答不同而都有变化,但免疫后1、6、15个月时的三次检测显示,免疫组的IgG抗体水平均显著高于对照组.免疫组小鼠1和15个月时的IgG抗体水平显著高于6个月时,而对照组间则无显著差异.唾液IgA抗Hf抗体
To understand whether long-term protection of co-immunized mice with both H. pylori (Hf) and cholera toxin resulted in long-term protection, the authors used 1 mg of sonicated Hf-mycobacteria on days 1, 3, 6, 30, Male BALB / c mice of 7-8 weeks old were challenged with live Hf via Hf at 1, 3, 6, 13 and 15 months after the last immunization, and the animals were sacrificed 21 days after the challenge and their stomach tissues The urease test and Giemsa staining were used to detect the presence of Helicobacter pylori, and the serum IgG and salivary IgA anti-Hf antibody were detected by ELISA.The results showed that all immunized mice at 1, 6, 13 and 15 months after immunization Was able to resist Hf challenge with an Hf clearance of 100% and a clearance of 86% (urease) or 93% (Giemsa method) at 3 months.Compared with the control group injected with saline, the clearance rate between the two groups There was a significant difference between the immune group and control group IgG antibody levels vary with the individual immune response have changed, but at 1, 6, 15 months after immunization three tests showed that the immune group IgG antibody levels were significantly higher than the control Group.The levels of IgG antibodies in the immunized mice at 1 and 15 months were significantly higher than those at 6 months while there was no significant difference between the control groups.Antibody IgA anti-Hf Body