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The effects of 5 mg/L 1,2,4-trichlorobenzene (TCB) and 0.1 mmol/L mercury ion (Hg2+) stresses on Ca2+ fluxion and protein phosphorylation in rice seedlings were investigated by isotope exchange kinetics and in vitro phosphorylation assay. The Ca2+ absorption in rice leaves and Ca2+ transportation from roots to leaves were promoted significantly in response to Hg2+ and TCB treatments for 4-48 h. The Ca2+ absorption peaks presented in the leaves when the rice seedlings were exposed to Hg2+ for 8-12 h or to TCB for 12-24 h. Several Ca2+ absorption peaks presented in the roots during rice seedlings being exposed to Hg2+ and TCB, and the first Ca2+ absorption peak was at 8 h after being exposed to Hg2+ and TCB. The result of isotope exchange kinetic analysis confirmed that short-term (8 h) Hg2+ and TCB stresses caused Ca2+ channels or pumps located on plasmalemma to open transiently. The phosphorylation assay showed that short-term TCB stress enhanced protein phosphorylation in rice roots (TCB treatment for 4-8 h) and leaves (TCB treatment for 4-24 h), and short-term (4-8 h) Hg2+ stress also enhanced protein phosphorylation in rice leaves. The enhancement of protein phosphorylation in both roots and leaves corresponded with the first Ca2+ absorption peak, which confirmed that the enhancement of protein phosphorylation caused by TCB or Hg2+ stress might be partly triggered by the increases of cytosolic calcium. TCB treatment over 12 h inhibited protein phosphorylation in rice roots, which might be partly due to that TCB stress suppressed the protein kinase activity. Whereas, Hg2+ treatment inhibited protein phosphorylation in rice roots, and Hg2+ treatment over 12 h inhibited protein phosphorylation in rice leaves. This might be attributed to that not only the protein kinase activity, but also the expressions of phosphorylation proteins were restrained by Hg2+ stress.
The effects of 5 mg / L 1,2,4-trichlorobenzene (TCB) and 0.1 mmol / L mercury ion (Hg2 +) on Ca2 + fluxion and protein phosphorylation in rice seedlings were investigated by isotope exchange kinetics and in vitro phosphorylation assay. Ca2 + absorption in rice leaves and Ca2 + transportation from roots to leaves were promoted significantly in response to Hg2 + and TCB treatments for 4-48 h. The Ca2 + absorption peaks presented in the leaves when the rice seedlings were exposed to Hg2 + for 8-12 h or to TCB for 12-24 h. Several Ca2 + absorption peaks presented in the roots during rice seedlings being exposed to Hg2 + and TCB, and the first Ca2 + absorption peak was at 8 h after being exposed to Hg2 + and TCB. The result of isotope exchange kinetic The phosphorylation assay showed that short-term TCB stress enhanced protein phosphorylation in rice roots (8 h) Hg2 + and TCB-induced Ca2 + channels or pumps located on plasmalemma to open transiently. TCB treatment for 4-8 h) and leaves (TCB treatment for 4-24 h), and short-term (4-8 h) Hg2 + stress also enhanced protein phosphorylation in rice leaves. The enhancement of protein phosphorylation in both roots and leaves which were caused by the increase of protein phosphorylation caused by TCB or Hg2 + stress might be slightly triggered by the increase of cytosolic calcium. which might be partly due Whereas, Hg2 + treatment inhibited protein phosphorylation in rice roots, and Hg2 + treatment over 12 h inhibited protein phosphorylation in rice leaves. This might be attributed to that not only the protein kinase activity, but also the expressions of phosphorylation proteins were restrained by Hg2 + stress.