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感受痛觉和触觉的背根神经节(DRG)神经元,不仅仅接受来自内部和外部的感觉刺激,还接受其他神经元的输入。然而,关于输入神经元的来源,至今没有系统地阐释。本实验旨在探讨初级感觉神经元的局部和下行输入来源。方法:(1)转基因小鼠:RΦGT小鼠表达Cre依赖性的rabies-G protein(狂犬病毒G蛋白)。将RΦGT小鼠与Avil-Cre小鼠和Trpv1-Cre小鼠分别杂交,获得Avil-Cre RΦGT和Trpv1-Cre RΦGT两种转
Dorsal root ganglion (DRG) neurons that feel pain and tactile sensations, not only receiving sensory stimuli from inside and outside, but also from other neurons. However, the source of input neurons has so far not been systematically elucidated. This experiment aims to investigate the primary and downstream input sources of primary sensory neurons. Methods: (1) Transgenic mice: RΦGT mice express Cre-dependent rabies-G protein. The RΦGT mice were respectively crossed with Avil-Cre mice and Trpv1-Cre mice to obtain two types of transformation of Avil-Cre RΦGT and Trpv1-Cre RΦGT