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目的分析耐亚胺培南肺炎克雷伯耐药基因检测效果,并对耐药机制进行分析。方法选取近3年分离出的260株耐亚胺培南肺炎克雷伯菌,采用新型耐药基因检查方法,对药物浓度进行分析。结果 Hodge检查结果显示,260株耐亚胺培南肺炎克雷伯菌中240株检查结果为阳性,20株为阴性,阳性率为92.3%。对β-内酰胺酶基因分析显示,有225株耐亚胺培南肺炎克雷伯菌产生KPC-2型碳青霉烯酶,其余耐亚胺培南肺炎克雷伯菌扩增出SHV型β-内酰胺酶基因。结论耐亚胺培南肺炎克雷伯菌的衍生因素比较复杂,在实践阶段需要做好耐药基因检查工作,只有做好检测工作,才能确定病菌类型。
Objective To analyze the detection results of Klebsiella pneumoniae resistant to imipenem and to analyze the mechanism of drug resistance. Methods 260 strains of Klebsiella pneumoniae resistant to imipenem isolated in the recent 3 years were selected and drug resistance was analyzed by a novel drug resistance gene test. Results The results of Hodge test showed that 240 out of 260 imipenem - resistant Klebsiella pneumoniae were positive and 20 were negative, the positive rate was 92.3%. The β-lactamase gene analysis showed that there were 225 strains of Klebsiella pneumoniae producing KPC-2 carbapenemase, and the other strains resistant to imipenem-producing Klebsiella pneumoniae amplified SHV type β-lactamase gene. Conclusions The imipenem-producing Klebsiella pneumoniae is more complicated in derivation, and it is necessary to carry out the drug resistance gene check in the practice stage. Only by doing the testing, can the type of bacteria be determined.