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为了解浙江省新昌县婴幼儿病毒性腹泻的病原学特征,制定有效防治对策,新昌县疾病预防控制中心采集新昌县某医院2010-2012年5~12月份婴幼儿腹泻患者的粪便样本546份,采用实时荧光定量-聚合酶链式反应同时进行轮状病毒、诺如病毒和肠道腺病毒的核酸检测。结果显示:在546份粪便样本中,263份样本检出至少1种病毒核酸,核酸阳性检出率为48.17%(263/546),轮状病毒、诺如病毒和肠道腺病毒的检出率分别为22.71%(124/546)、13.74%(75/546)和20.51%(112/546),混合感染占8.24%(45/546),病毒检出主要集中于2岁以下儿童腹泻患者。监测结果表明病毒为本地区婴幼儿腹泻的重要病因,其中轮状病毒和肠道腺病毒均为最主要的病原体,其次为诺如病毒。对混合感染现象需要引起重视。
In order to find out the etiological characteristics of infantile diarrhea in Xinchang County of Zhejiang Province and establish effective prevention and control measures, Xinchang CDC collected 546 stool samples of infants and young children with diarrhea from 2010 to 2012 in Xinchang County from 2010 to 2012, Real-time fluorescence quantitative polymerase chain reaction (PCR) was used to detect the nucleic acids of rotavirus, norovirus and enterovirus simultaneously. The results showed that in 546 stool samples, at least one virus nucleic acid was detected in 263 samples, the positive rate of nucleic acid was 48.17% (263/546), the detection of rotavirus, norovirus and enterovirus The rates of virus infection were 22.71% (124/546), 13.74% (75/546) and 20.51% (112/546) respectively, and the mixed infections accounted for 8.24% (45/546). The detection of the virus mainly focused on diarrhea patients under 2 years of age . Monitoring results show that the virus is an important cause of diarrhea in infants and young children in the region, of which rotavirus and enteric adenovirus are the most important pathogens, followed by Norovirus. The phenomenon of mixed infection needs attention.