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目的:设计一种用于检测CYP3A5基因分型的电化学传感器阵列及其不同基因型的判别方法。方法:设计的电化学基体由印刷电路板(PCB)组成,该电路板包含一组金电极。每个金电极表面修饰有包含单链捕获探针的自组装单分子膜。设计中使用二茂铁做为电活性指示剂,基因分型检测是通过两种不同电势的二茂铁衍生物分别标记等位基因特异性信号探针来实现。结果:该设计能构建一种快速准确、操作简便的DNA电化学传感器阵列检测系统。结论:本文设计为使用电化学方法检测基因分型提供了一种新方法和新技术。
OBJECTIVE: To design an electrochemical sensor array for the detection of CYP3A5 genotyping and its discriminating method for different genotypes. Method: The designed electrochemical substrate consists of a Printed Circuit Board (PCB), which contains a set of gold electrodes. Each gold electrode surface is modified with a self-assembled monolayer containing a single-stranded capture probe. Ferrocene was used as an electroactive indicator in the design. Genotyping was performed using two different potentials of ferrocene derivatives labeled with allele-specific signal probes. Results: This design enables the construction of a rapid, accurate and easy-to-use DNA electrochemical sensor array detection system. Conclusion: This article was designed to provide a new method and new technique for the detection of genotyping using electrochemical methods.