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目的观察Osterix在调控脊椎松质骨骨量的作用及机制。方法以Osterix敲除小鼠和Osterix转基因小鼠为观察对象,采用X线摄像、显微电子计算机X线断层摄影术(Micro-CT)和苏木素-伊红(HE)染色观察Osterix过表达或敲除后小鼠腰椎骨量的变化,抗酒石酸酸性磷酸酶(TRAP)染色观察破骨细胞的变化。免疫组织化学检测核因子-κB受体活化因子配体(RANKL)表达情况的变化。结果 Osterix转基因小鼠腰椎骨量没有明显变化,而X线片、Micro-CT和HE染色检测显示Osterix敲除小鼠在出生后12周脊椎密度和骨量增加;TRAP染色显示腰椎中破骨细胞数量减少;免疫组织化学染色检测显示其椎体中RANKL表达水平降低。结论 Osterix在调控脊椎松质骨骨量中起到重要作用。
Objective To observe the effect and mechanism of Osterix in the regulation of spine cancellous bone. Methods Osterix knockout mice and Osterix transgenic mice were used as the observation objects. Osterix overexpression or knocking was observed by X-ray, Micro-CT and HE staining. Except for the change of lumbar vertebrae bone mass, the changes of osteoclasts were observed by tartrate-resistant acid phosphatase (TRAP) staining. Immunohistochemical detection of nuclear factor-kappa B receptor activator ligand (RANKL) expression changes. Results Osterix transgenic mice had no significant changes in lumbar bone mass. The X-ray, Micro-CT and HE staining showed that the density and bone mass of Osterix knockout mice increased 12 weeks after birth. TRAP staining showed that osteoclasts The quantity decreased; the immunohistochemical staining showed that the expression of RANKL in vertebral body decreased. Conclusion Osterix plays an important role in the regulation of spine cancellous bone mass.