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目的 观察COPD患者诱导痰细胞学和痰上清液IL -8浓度的变化 ,探讨诱导痰在COPD气道炎症监测中的作用。方法 以 2 6例临床确诊的稳定期COPD患者为研究对象 ,分离诱导痰细胞 ,同时检测痰上清液IL -8浓度和肺功能。并以 15例健康志愿者作为对照组。结果 COPD稳定期组与正常对照组诱导痰细胞总数、巨噬细胞数及中性粒细胞数比较 ,差异有显著性 (P <0 0 1) ;COPD稳定期组中性粒细胞百分率高于正常组 ,(P <0 0 1)。稳定期组诱导痰上清液IL -8浓度与正常组比较差异有显著性(P <0 0 1)。诱导痰上清液IL -8的浓度与FEV1占预计值 %呈负相关关系 (r=-0 5 3 9,P <0 0 1) ;诱导痰PMN数与FEV1占预计值 %呈负相关关系 (r =-0 73 3 ,P <0 0 1) ;AM数与FEV1占预计值 %无相关关系 (r =-0 2 2 3 ,P =0 161)。结论 诱导痰技术无创、简便 ,是监测COPD气道炎症的较理想指标。
Objective To observe the change of induced sputum cytology and IL - 8 concentration in sputum supernatant of patients with chronic obstructive pulmonary disease (COPD) and explore the role of induced sputum in the monitoring of airway inflammation in COPD patients. Methods Twenty - six patients with stable COPD clinically diagnosed were divided into induced sputum cells and IL - 8 concentration and pulmonary function in sputum supernatant. Fifteen healthy volunteers were used as control group. Results The total number of sputum cells, the number of macrophages and neutrophils induced in stable COPD group and normal control group were significantly different (P <0.01). The percentage of neutrophils in stable COPD group was higher than that in normal group Group, (P <0 0 1). The concentration of IL-8 in induced sputum supernatant of stable group was significantly different from that in normal group (P <0.01). The concentration of IL-8 in induced sputum supernatant was negatively correlated with the predicted value of FEV1 (r = -0 5 3 9, P <0.01); the number of induced sputum PMN was negatively correlated with the predicted value of FEV1 (r = -073 3, P <0.01). There was no correlation between the number of AM and the predicted value of FEV1 (r = -0223, P = 0161). Conclusion Induced sputum technique is noninvasive and simple, it is a more ideal indicator to monitor airway inflammation of COPD.