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铜是生物体必需的微量金属元素,它是很多氧化还原酶的必要组成成份,又是机体组织某些病变的指示剂。根据变因学的调查研究,发现在肝癌高发区,环境中铜锌含量偏高。而在化学元素中,铜是优先富集于肝脏组织的元素之一。因此,人类生存环境中微量铜的测定和人类自身发展,有着密切的联系。 4.5-二溴苯基荧光酮(DBPF),在表面活性剂存在下,是近几年来应用甚为广泛的高灵敏显色剂,已用于很多高价金属离子的测定。但利用Cu(Ⅱ)与DBPF在表面活性剂存在下的络合体系却未见报道。本文研究了在溴化十六烷基三甲铵(CTMAB)存在下吸光光度法测定铜(Ⅱ)的有关条件,可用于矿泉水、血清中微量铜的测定。该法操作简便、方法灵敏、准确。
Copper is an essential trace metal element of the organism, which is an essential component of many oxidoreductases and an indicator of certain lesions in body tissues. According to the study of etiology, it was found that in areas with high incidence of liver cancer, copper and zinc contents were high in the environment. Among the chemical elements, copper is one of the elements preferentially enriched in liver tissue. Therefore, there is a close relationship between the determination of trace copper in human living environment and the development of human beings themselves. DBPF, in the presence of surfactants, is a highly sensitive developer widely used in recent years and has been used in the determination of many high-valent metal ions. However, the use of Cu (Ⅱ) complex with DBPF in the presence of surfactants has not been reported. In this paper, the conditions for the determination of copper (Ⅱ) by spectrophotometry in the presence of cetyltrimethylammonium bromide (CTMAB) were studied. It can be used for the determination of trace copper in mineral water and serum. The method is simple, sensitive and accurate.