Preparation of Anti-HER2 Monoclonal Antibody-paclitaxel Immunoconjugate and Its Biological Evaluatio

• 作 者: Dong LIU Yanjiao XU Zichao RAO Zhaocong CHEN
• 机 构: Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and
• 出 处: 华中科技大学学报（医学）（英德文版）
• 发表日期: 2011年6期
• 关键词: paclitaxel anti-HER2 monoclonal antibody immunoconjugate specific binding

Anti-HER2 monoclonal antibody (Sc7301)-paclitaxel (TAX) immunoconjugate was prepared and its specific binding to tumor cells was investigated in this study.Sc7301 was conjugated to　TAX by the active ester method and then the TAX-Sc7301 immunoconjugate was obtained.After purification and labeling by Cyano-fluorescein isothiocyanate (FITC),the specific binding of TAX-Sc7301to HER2-positive tumor cells (SKOV3) and HER2-negative tumor cells (HepG2) was evaluated respectively.TAX-Sc7301 (20 nmol/L) showed distinct specific binding to SKOV3 cells rather than HepG2cells.And the uptake of the immunoconjugate by SKOV3 cells was increased with the TAX-Sc7301concentration (3-48 nmol/L) and the incubation time (P＜0.05).It was concluded that the TAX-Sc7301immunoconjugate is potentially applicable as a targeted agent against HER2-positive tumor cells.