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目的改进《血铅临床检验技术规范》(简称规范)推荐的GFAAS测定血铅的方法,提高灵敏度和准确度,使该法应用更为可行、可靠。方法用接近空白的基底血制备标准系列,用1%硝酸和含铵盐与曲拉通的化学基体改进剂(简称基改)稀释血样,与石墨炉原子吸收光谱测定血铅含量。结果血铅浓度0~80μg/L内,线性良好,回归方程y=0.007 14x+0.016 6,相关系数r为0.999 4,方法检出限1.7μg/L,定量检出限5.7μg/L,加标回收率97.6%~100.9%,精密度2.48%~5.69%。结论本法线性范围及相关系数、检出限、精密度、准确度、室内质控,参加省主管单位盲样考核合格,均符合《规范》的要求,适用于日常工作的开展。
Objective To improve the method of determination of blood lead by GFAAS recommended by the Technical Specification for Clinical Test of Blood Lead (referred to as “Specification for Blood Lead”) for more sensitivity and accuracy, making the method more feasible and reliable. Methods Standard series was prepared with nearly blank blood of the base. Blood samples were diluted with 1% nitric acid and a chemical base modifier containing ammonium salt and triptolide (referred to as base modification), and blood lead content was determined by graphite furnace atomic absorption spectrometry. Results The calibration curve was linear in the range of 0 ~ 80μg / L, the regression equation was y = 0.00714x + 0.016 6, the correlation coefficient was 0.999 4, the detection limit was 1.7μg / L and the limit of quantification was 5.7μg / L. The standard recovery of 97.6% ~ 100.9%, precision of 2.48% ~ 5.69%. Conclusion The linear range and correlation coefficient of this method, detection limit, precision, accuracy, indoor quality control, blind spot examination of participating provincial units are in conformity with the requirements of “norms” and are applicable to the daily work.