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目的 探讨抗坏血酸与乳腺癌MDA-MB-231细胞增殖和凋亡的相关性。方法分别用0.5、1.0、2.0和4.0mmol/L的抗坏血酸处理MDA-MB-231细胞,MTT法检测细胞增殖水平;RT-PCR检测细胞p53和bcl-2基因mRNA的转录水平;West-ern blot检测细胞P53和bcl-2蛋白的表达;流式细胞术检测细胞的凋亡率。结果不同浓度的抗坏血酸均可明显抑制MDA-MB-231细胞增殖,促进其凋亡,并使细胞p53基因mRNA转录水平及P53蛋白的表达水平明显升高,而bcl-2基因mRNA转录水平及bcl-2蛋白表达水平明显降低,且均呈剂量依赖性。结论抗坏血酸能抑制MDA-MB-231细胞增殖,诱导其凋亡,其机制可能是通过上调P53,下调bcl-2介导的。
Objective To investigate the correlation between ascorbic acid and proliferation and apoptosis of breast cancer MDA-MB-231 cells. Methods MDA-MB-231 cells were treated with 0.5, 1.0, 2.0 and 4.0 mmol / L ascorbic acid respectively. The cell proliferation was detected by MTT assay. The transcription level of p53 and bcl-2 mRNA was detected by RT- The expression of P53 and bcl-2 protein was detected by flow cytometry. The apoptosis rate was detected by flow cytometry. Results Different concentrations of ascorbic acid could significantly inhibit the proliferation and promote the apoptosis of MDA-MB-231 cells. The transcription level of p53 gene and the expression of P53 protein were significantly increased, while the mRNA and protein levels of bcl-2 mRNA and bcl- -2 protein expression was significantly reduced, and were dose-dependent. Conclusion Ascorbic acid can inhibit the proliferation and induce the apoptosis of MDA-MB-231 cells, which may be mediated through up-regulation of P53 and down-regulation of bcl-2.