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目的探讨茶多酚对高糖条件下培养的大鼠晶状体上皮细胞死亡率(CDR)及线粒体膜电位(MMP)的影响,阐明糖尿病性白内障的发生机制及茶多酚干预的疗效及机制。设计实验研究。研究对象体外培养的大鼠晶状体上皮细胞。方法体外培养大鼠晶状体上皮细胞,培养基中葡萄糖浓度分别为5、30mmol/L(L1组和L2组),另在30mmol/L葡萄糖浓度培养基中加入0.1μM和0.3μM的茶多酚进行干预(L3组和L4组)。流式细胞仪检测各条件下大鼠晶状体上皮细胞MMP和CDR的变化。主要指标大鼠晶状体上皮细胞CDR及MMP。结果L1、L2、L3、L4组大鼠晶状体上皮细胞CDR分别为(0.68±0.32)%、(13.50±4.48)%、(0.64±0.48)%、(0.50±0.30)%,L1与L2组比较、L2与L3组比较差异均有统计学意义(P=0.04,P=0.04),L3与L4组比较差异无统计学意义(P=0.99)。L1、L2、L3、L4组大鼠晶状体上皮细胞MMP分别为95.53±4.61、37.38±3.56、110.02±8.04、102.63±9.48,L1与L2组比较、L2与L3组比较差异均有统计学意义(P=0.01,P=0.01),L3与L4组比较差异无统计学意义(P=1.00)。结论高糖条件下培养的大鼠晶状体上皮细胞线粒体膜电位降低、细胞死亡率明显升高。茶多酚具有稳定线粒体膜电位的作用,可延缓细胞凋亡,降低细胞死亡率。
Objective To investigate the effects of tea polyphenols on rat lens epithelial cell death (CDR) and mitochondrial membrane potential (MMP) under high glucose conditions, clarify the mechanism of diabetic cataract and the efficacy and mechanism of tea polyphenols intervention. Design experimental studies. The subject was cultured in vitro with rat lens epithelial cells. Methods Rat lens epithelial cells were cultured in vitro. The concentration of glucose in culture medium was 5 and 30 mmol/L (L1 group and L2 group), respectively, and 30 μg/L glucose concentration medium was added with 0.1 μM and 0.3 μM tea polyphenols. Interventions (L3 group and L4 group). The changes of MMP and CDR in rat lens epithelial cells were detected by flow cytometry. The main indicators of rat lens epithelial cells CDR and MMP. RESULTS: The CDRs of lens epithelial cells in L1, L2, L3, and L4 groups were (0.68±0.32)%, (13.50±4.48)%, (0.64±0.48)%, (0.50±0.30)%, respectively, compared with L1 and L2 groups. There was significant difference between L2 and L3 groups (P=0.04, P=0.04). There was no significant difference between L3 and L4 groups (P=0.99). The MMP levels of L1, L2, L3, and L4 lens epithelial cells were 95.53±4.61, 37.38±3.56, 110.02±8.04, and 102.63±9.48, respectively. There was significant difference between L1 and L2 groups and between L2 and L3 groups. P=0.01, P=0.01), there was no significant difference between L3 and L4 groups (P=1.00). Conclusion The mitochondrial membrane potential of rat lens epithelial cells cultured under high glucose conditions was decreased and the cell death rate was significantly increased. Tea polyphenols have the effect of stabilizing the mitochondrial membrane potential, which can delay apoptosis and reduce cell death.