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目的:探讨外周炎症损伤后诱致脊髓背角Ⅰ层神经元功能可塑性和结构可塑性改变的情况。方法:采用脊髓薄片全细胞膜片钳记录技术和Golgi染色方法。结果:小鼠一侧后肢足底皮下注射完全弗氏佐剂(complete freund’s adjuvant,CFA)可诱致显著的机械性痛敏和热痛敏。CFA注射24 h后,对脊髓背角Ⅰ层神经元进行全细胞膜片钳记录发现刺激伤害性初级传入纤维诱致的兴奋性突触后电流(excitatory postsynaptic currents,EPSCs)的幅值较对照组动物显著增加,平均增强幅度为85.7%。Golgi染色结果发现,在诱致功能可塑性增强的同时,CFA炎症后还可导致脊髓背角Ⅰ层神经元出现树突棘密度显著增加,与对照组相比平均增强186.7%。结论:CFA引发的外周炎症损伤可同时诱致脊髓背角Ⅰ层神经元呈现功能可塑性和结构可塑性增强,这些变化与疼痛的持续慢性化过程有关。
OBJECTIVE: To investigate the changes of functional plasticity and structural plasticity of layer I neurons in the dorsal horn of spinal cord after peripheral inflammation injury. Methods: Whole-cell patch-clamp recording technique and Golgi staining method were used. RESULTS: Subcutaneous injection of complete freund’s adjuvant (CFA) on the hindlimb foot of mice induced significant mechanical and thermal hyperalgesia. Whole-cell patch-clamp recordings of neurons in the dorsal horn of spinal cord 24 h after CFA injection showed that amplitude of excitatory postsynaptic currents (EPSCs) stimulated by nociceptive primary afferent fibers was significantly higher than that of control animals Significant increase, the average increase of 85.7%. The results of Golgi staining showed that, after inducing the enhancement of functional plasticity, the inflammation of CFA induced a significant increase of density of dendritic spines in the neurons of layer Ⅰ of spinal dorsal horn, which was increased by 186.7% compared with the control group. CONCLUSION: Peripheral inflammatory injury induced by CFA can induce the functional plasticity and structural plasticity of Ⅰ layer neurons in spinal dorsal horn. These changes are related to the continuous chronic pain process.