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流行性乙型脑炎(简称乙脑)和基孔肯雅病(Chikungunya)均由病毒引起,经蚊虫传播,严重危害人类健康的自然疫源性疾病。为进一步了解乙脑病毒和基孔肯雅病毒的宿主情况,我们用血凝抑制试验对采自云南的蝙蝠和恒河猴血清中进行了乙脑及基孔肯雅病毒抗体的检测。现将结果报告如下。1 材料与方法1.1 血清采集:棕果编蝠(Rousettusleschenaulti)血清,采自云南省河口地区。恒河猴(Macaca mulatta)血清,采自西双版纳、临沧及思茅地区。采到的动物血清—30℃低温保存,以备检测。1.2 试验方法:采用微量血凝制试验。乙脑和基孔肯雅抗原及免疫血清由本实验室自制,抗原用4个血凝单位,用0.5%的鸽血球测定。
Both Japanese encephalitis (JE) and Chikungunya are virus-borne, mosquito-borne, natural-borne diseases that seriously endanger human health. To further understand the host situation of JE virus and Chikungunya virus, we tested the JE and Chikungunya antibodies against the hemagglutination inhibition test in bats and rhesus monkeys collected from Yunnan. The results reported below. 1 Materials and Methods 1.1 Serum collection: Brown-eared bat (Rousettusleschenaulti) serum collected from the estuary of Yunnan Province. Macaca mulatta serum collected from Xishuangbanna, Lincang and Simao areas. The animal serum collected at -30 ℃ cryogenic preservation, ready for testing. 1.2 Test Method: Using trace hemagglutination test. JE and chikungunya antigens and immune sera are self-made in this laboratory. Antigen is measured in 4 hemagglutination units using 0.5% pigeon blood cells.