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目的探讨生长发育阶段,早期应激影响C57幼鼠发育的短期实验研究。方法利用早年生活应激的动物模型干预C57BL/6J母鼠与其幼崽,观察经出生后第2天给予持续早期的慢性应激的C57BL/6J幼鼠,并于第9日龄(P9)、3周龄(P21)时间点检测P 2~9 d幼鼠体质量增加情况;在P 2~9 d期间,每日相同时间段监测1 h内母鼠与幼崽的哺乳情况,幼崽离巢时间及母鼠离巢的次数;在P9、P21日清晨,C57BL/6J幼鼠称质量后颈椎脱臼牺牲幼鼠,分离幼鼠全脑、胸腺及双侧肾上腺并称质量。结果 1经早期慢性应激刺激的C57BL/6J幼鼠,1h观察时间内,每笼至少1只幼崽离巢时间明显高于正常喂养同龄组幼鼠(P<0.05),相同观察时间内母鼠出巢的次数明显高于对照组(P<0.05),而1 h观察时间内,母鼠-幼崽的哺乳时间差异无统计学意义(P>0.1);2经早期慢性应激刺激组幼鼠,在P 2~9 d体质量增加明显低于正常喂养组(P<0.05),在P9和P21 d,应激组幼鼠的胸腺与体质量比明显低于非应激组(P<0.05),而双侧肾上腺与体质量比差异无统计学意义(P>0.05)。结论发育早期的慢性应激可诱导C57小鼠胸腺过早萎缩。
Objective To investigate the short-term experimental study on the effects of early stage stress on the development of C57 young rats during growth and development. Methods C57BL / 6J female rats and their pups were intervened by animal model of early life stress. C57BL / 6J young rats, who were given continuous early chronic stress on the second day after birth, were observed. On the ninth day (P9), At 3 weeks of age (P21), the body weight of pups and pups in P 2 ~ 9 d were detected. During the same period of P 2 ~ 9 d, Nest time and the number of mothers leaving the nest; at P9, P21 day, C57BL / 6J baby rats were sacrificed after cervical vertebra dislocation, the whole brain, thymus and bilateral adrenal were isolated and weighed. Results 1 In the 1h observation period, at least 1 pup per cage in the C57BL / 6J young rats stimulated by early chronic stress was significantly longer than that in the normal fed group (P <0.05), and the same observation time (P <0.05). However, there was no significant difference in the lactation time between the two groups (P> 0.05) .2 After the early chronic stress stimulation group Compared with normal group, the increase of body weight of pups in P 2 ~ 9 d group was significantly lower than that in normal group (P <0.05). At P 9 and P 21, the thymus weight ratio of young rats was significantly lower than that of non-stressed group <0.05), while there was no significant difference in bilateral adrenal and body mass ratio (P> 0.05). Conclusion Chronic stress in early development induces premature atrophy of thymus in C57 mice.