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目的:探讨三磷酸腺苷-氯化镁(ATP-MgCl2)对高氧诱发新生鼠肺损伤的影响。方法:选用生后2天体重5～10 g的Wistar大鼠120只,随机分为空气对照组、高氧模型组、ATP-MgCl2治疗组,每组40只。新生鼠制备成高氧模型后第2天始治疗组新生鼠腹腔内注射ATP-MgCl2(45 mg/kg),空气对照组和高氧模型组腹腔内注射等量生理盐水,以上3组实验开始第3、7及14天每组处死10只动物,取肺组织,光镜下观察肺组织的病理变化,采用免疫组化染色观察肺组织中肿瘤坏死因子-a(TNF-a),转化生长因子(TGF-β1)和波形蛋白(Vimentin)的表达。结果:高氧模型组TNF-a、TGF-β1及Vim-entin表达强度明显高于空气对照组,差异有统计学意义(P<0.01)。ATP-MgCl2组TNF-a、TGF-β1及Vimentin表达强度低于高氧模型组,差异有统计学意义(P<0.05)。结论:三磷酸腺苷-氯化镁对新生大鼠高氧肺损伤有保护作用,对支气管肺发育不良有一定的预防作用。
Objective: To investigate the effect of adenosine triphosphate (ATP-MgCl2) on lung injury in neonatal rats induced by hyperoxia. Methods: 120 Wistar rats weighing 5 ~ 10 g 2 days after birth were randomly divided into three groups: air control group, hyperoxia model group and ATP-MgCl2 treatment group. The neonatal rats were injected intraperitoneally with ATP-MgCl2 (45 mg / kg) on the second day after the preparation of hyperoxia model. The rats in the air control group and the hyperoxia group were injected intraperitoneally with the same amount of saline, and the above three groups of experiments started On the 3rd, 7th and 14th day, 10 animals in each group were sacrificed and the lung tissue was taken out. The pathological changes of lung tissue were observed under light microscope. The expression of tumor necrosis factor-a (TNF-a) in lung tissue was observed by immunohistochemistry, Factor (TGF-β1) and vimentin (Vimentin) expression. Results: The expression of TNF-a, TGF-β1 and Vim-entin in hyperoxia model group was significantly higher than that in air control group, the difference was statistically significant (P <0.01). The expression of TNF-a, TGF-β1 and Vimentin in ATP-MgCl2 group was lower than that in hyperoxia model group (P <0.05). CONCLUSION: Adenosine triphosphate - magnesium chloride has protective effect on hyperoxia - induced lung injury in neonatal rats, and has some preventive effects on the development of bronchopulmonary dysplasia.