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高效液相色谱法测定人体液胆汁酸常遇到干扰多、检测特异性差的问题。为此我们曾在1989年报道用固相酶检测系统测定胆汁酸。该方法虽然灵敏度高、特异性强,但方法难度大,普通实验室难以推广。二极管阵列检测器是一种应用于色谱分析的新型的紫外—可见分光鉴定器。该检测器能在50ms 内完成光谱扫描,又色谱峰洗脱每瞬间都能提供一张全光谱图,当某一组分色谱峰各点光谱图结构完全一致,即可判断该组分是纯物质。因而二极管阵列检测器有较强的定性能力和较高的灵敏度。我们选用该检测器紫外
The determination of bile acids in human fluids by high performance liquid chromatography often encounters many problems of interference and poor specificity. For this reason, we reported in 1989 that solid-phase enzyme detection systems were used to measure bile acids. Although this method has high sensitivity and specificity, it is difficult for the method to be popularized by ordinary laboratories. The diode array detector is a new type of UV-visible spectrometer for chromatographic analysis. The detector can complete the spectral scanning within 50ms, and the chromatogram peak can provide a full spectrum at every moment. When the spectrum of each component of the chromatogram peak is completely the same, it can be judged that the composition is pure. substance. Therefore, the diode array detector has strong qualitative ability and high sensitivity. We use this detector UV