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一、引言DNA限制性片段长度多态性(Restriction Fragment Length Polymorphism简称RFLP)是近年来分子遗传学研究中发展起来的一种新技术。其技术要点是:DNA经限制性内切酶酶切后,产生不同长度的片段;经琼脂糖电泳分离这些不同的片段,印迹转移至硝酸纤维素滤膜上;然后用一放射性同位素标记的特定DNA克隆(探针)杂
I. INTRODUCTION Restrictive Fragment Length Polymorphism (RFLP) is a new technique developed in molecular genetics in recent years. The technical points are: DNA is digested with restriction endonuclease to generate fragments of different lengths; these different fragments are separated by agarose electrophoresis and imprinted onto a nitrocellulose filter; and then a radioisotope-labeled specific DNA Cloning (Probe) Miscellaneous