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提出一种应用于心肌肌钙蛋白Ⅰ(cTn Ⅰ)检测的局域表面等离子共振(LSPR)光纤传感器。将标准光纤局部腐蚀掉包层,在其表面形成纳米银膜,用葡萄球菌A蛋白(SPA)作为纳米银膜与cTn Ⅰ单克隆抗体的连接介体,实现鼠抗人cTn Ⅰ单克隆抗体对光纤传感器的修饰,放入不同浓度的cTn Ⅰ溶液中,通过测量传感器光谱曲线的消光峰位移获得溶液中的cTn I浓度。实验结果表明,溶液中cTn Ⅰ浓度在20～120ng/mL范围内,光谱曲线的消光峰位移的对数与cTn Ⅰ质量浓度的对数成线性关系,线性相关系数为0.996 2。利用夹心法,传感器检测限可达10ng/mL,有较强的抗杂蛋白干扰能力。基于LSPR的光纤传感器件结构简单、性能稳定、成本低且测量灵敏度高,可应用于cTn Ⅰ检测。
A localized surface plasmon resonance (LSPR) optical fiber sensor for detection of cardiac troponin Ⅰ (cTn Ⅰ) was proposed. The standard optical fiber is locally etched off to form a nano-silver film on the surface of the optical fiber. Staphylococcus A protein (SPA) is used as a connecting mediator of the nano-silver film and the cTnI monoclonal antibody to achieve the effect of the mouse anti-human cTnI monoclonal antibody on the optical fiber The sensor was modified and placed in different concentrations of cTn I solution. The concentration of cTn I in the solution was obtained by measuring the extinction peak shift of the sensor spectral curve. The experimental results show that the logarithm of the extinction peak of the spectral curve is linear with the logarithm of the mass concentration of cTn Ⅰ, and the linear correlation coefficient is 0.996 2 in the range of 20 ~ 120 ng / mL. Using the sandwich method, the detection limit of the sensor can reach 10ng / mL, which has a strong ability to resist the interference of the miscellaneous proteins. The LSPR-based optical fiber sensing device has the advantages of simple structure, stable performance, low cost and high measurement sensitivity, and can be applied to cTn Ⅰ detection.