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目的 :克隆小鼠CD2 2 6 (PTA1)分子。方法 :从GenBank中检索出与人CD2 2 6分子在氨基酸水平上有 5 1%同源性的EST序列 ,设计并合成特异性引物 ,采用快速扩增cDNA末端的RACE方法 ,从 4周龄BALB c小鼠的胸腺中扩增小鼠CD2 2 6cDNA序列。结果 :克隆出完整的小鼠CD2 2 6cDNA ,长 2 2 2 3bp ,其中开放读框为 10 0 2bp ,编码含信号肽在内的 333个氨基酸 ,属免疫球蛋白超家族分子 ,较人CD2 2 6分子少了 3个氨基酸 ,在氨基酸水平上有 5 3%的同源性。此外 ,还克隆了小鼠CD2 2 6分子的 3种异型。结论 :成功克隆出小鼠CD2 2 6 (PTA1)cDNA ,并发现 3种异型 ,全面探讨该分子生物学特性 ,进行体内功能实验 ,基因敲除小鼠和转基因小鼠的研究提供了坚实的基础。
Objective: To clone murine CD2 2 6 (PTA1) molecule. METHODS: EST sequences homologous to human CD2 2 6 at the amino acid level were retrieved from GenBank. Specific primers were designed and synthesized. The rapid amplification of cDNA ends of RACE was used to detect the expression levels of 4-week-old BALB / c mice mouse CD2 26 cDNA sequence was amplified in the thymus of c mice. Results: The complete mouse CD2 2 6 cDNA was cloned, with a length of 2222 bp and an open reading frame of 10 bp. The 333 amino acids encoding the signal peptide, belonging to the immunoglobulin superfamily, 6 molecules less 3 amino acids, 5 3% homology at the amino acid level. In addition, three isoforms of murine CD22 6 molecules were cloned. CONCLUSION: CD2 2 6 (PTA1) cDNA was successfully cloned and found three kinds of abnormal, fully explored the molecular biological characteristics, in vivo functional experiments, knockout mice and transgenic mice provide a solid foundation .