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通过剪顶包扎菌液接种、伤根淋灌菌液接种、 WFT培养接种测定结果与桉树当年新造林分青枯病自然发生率调查结果对比 ,青枯病菌对桉苗的致病力因接种方法不同而异 ,经模糊聚类分析表明 ,WFT接菌培养技术测出的结果更接近自然实际发病情况。试验表明 ,桉树不同品种、同一品种的不同种源、不同无性系 ,对青枯病菌的抗性不同。课题组通过 8年研究 ,在生产单位提供的 10 1个桉树品系中 ,已筛选出高抗品系 12个、抗病品系 14个、低感品系 2 6个。自 1994年起在粤、桂、琼三省区 ,生产上大面积推广这些高抗和抗病品 (种 )系 ,已直接造林 2万多 hm2 ,扩散面积更大 ,造林效果跟踪检查表明 ,这些林分的青枯病株率均在 1‰以下 ,受到生产者和经营者欢迎。试验和应用中发现部分抗病无性系随着在林木组培苗工厂连续繁殖使用年份增加其抗病性逐年出现下降 ,有些抗病无性系使用 3年以后 ,其抗病性就明显下降 ,值得引起重视
Inoculation by clipping the top of the banding bacteria, root canal irrigation liquid injury inoculation, WFT inoculation test results and eucalyptus afforestation new occurrence of bacterial wilt compared to the natural incidence of bacterial pathogen of Eucalyptus seedlings by inoculation method Different fuzzy clustering analysis shows that WFT inoculation culture technology results closer to the actual incidence of the actual situation. Tests showed that different varieties of eucalyptus, different provenances of the same variety, different clones, different resistance to bacterial wilt. After 8 years of research, the research group has selected 12 highly resistant lines, 14 resistant lines and 26 low susceptible lines out of the 101 eucalyptus lines provided by the production units. Since 1994, Guangdong, Guangxi and Qiong provinces have been promoting large-scale production of these high-resistance and disease-resistant products (strains), and have directly afforestation of more than 20,000 hm2 with more spreading area. The tracking inspection of afforestation results shows that these The rate of bacterial wilt in stands is below 1 ‰, which is welcomed by producers and operators. Some resistant clones were found in the experiment and application. With the year of continuous propagation in tree tissue culture plants, their disease resistance decreased year by year. Some disease-resistant clones declined significantly after 3 years of use, Caused attention