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目的 探讨干扰素α治疗慢性乙型肝炎过程中ＩＬ－１２对其外周血单个核细胞（ＰＢＭＣ）产生 ＩＦＮ γ和 ＩＬ—１０的动态影响。方法 用 ＥＬＩＳＡ法分别检测 ２０例慢性乙型肝炎患者在干扰素α２ｂ治疗前、治疗 ３个月、治疗 ６个月时 ＰＢＭＣ在 ＰＨＡ（１００μｇ／ｍｌ）、ＨＢｃＡｇ（１μｇ／ｍｌ）、ＨＢｅＡｇ（１μｇ／ｍｌ）单独或联合 ＩＬ—１２（１０ｎｇ／ｍｌ）体外培养４８ｈ后培养上清液ＩＮＦγ和ＩＬ—１０的水平。结累 １２例干扰素应答患者联合ＩＬ—１２诱导，同一诱导组在治疗 ３个月和 ６个月同治疗前比较，ＩＦＮ γ水平明显增高（Ｐ＜０．０１），同一治疗阶段，单独抗原诱导和联合 ＩＬ—１２诱导相比，联台诱导组 ＩＦＮ γ水平明显增高（Ｐ＜０．０１），治疗 ３个月和 ６个月时，ＩＬ一１２对ＩＬ－１０ 的产生表现出明显的抑制作用（ＨＢｃＡｇ诱导组例外）。８例干扰素无应答患者联合ＩＬ—１２诱导，同一诱导组在治疗３个月和６个月同治疗前相比，ＩＦＮγ水平明显增高（Ｐ＜０．０１），同一治疗阶段，单独抗原诱导和联合ＩＬ－１２诱导相比，联合诱导组ＩＦＮ γ水平明显增高，并随治疗时间的延长，协同效应更为明显。结论 在干扰素治疗过程中，ＩＬ—１２对乙?
Objective To investigate the dynamic effects of IL-12 on the production of IFN-γ and IL-10 in peripheral blood mononuclear cells (PBMCs) during the treatment of chronic hepatitis B with interferon α. Methods Twenty patients with chronic hepatitis B were pretreated with interferon α2b for 3 months before treatment with ELISA. The PBMCs were cultured in PHA (100μg / ml), HBcAg (1μg / ml), HBeAg (1μg / ml) alone or in combination with IL-12 (10 ng / ml) for 48 h in vitro. In the same induction group, the level of IFN-γincreased significantly (P <0.01) in the same induction group at 12 months and 6 months after treatment with IFN- Compared with IL-12 induction, the level of IFN-γ in co-therapy group was significantly increased (P <0.01). At 3 months and 6 months, the production of IL-10 by IL-12 showed obvious Inhibition (exception to HBcAg induction group). In the same induction group, the level of IFNγ was significantly increased (P <0.01) in 8 patients who did not respond to interferon combined with IL-12. The levels of IFNγ were significantly increased in the same induction group at 3 and 6 months Compared with the combination of IL-12 induction, the IFN-γ level in the combined induction group was significantly increased, and with the extension of treatment time, the synergistic effect was more obvious. Conclusion In the process of interferon treatment, IL-12 on B?