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因为成年人和胎儿胰腺供体组织来源不足,而猪胚胎前胰岛具有种种固有的有利条件,所以近年来,猪胚胎前胰岛移植研究逐步引起重视。妊娠中期胎龄75±3天的绪胚胎的胰腺组织,经胶原酶轻度消化后,培养10天。经培养所形成的胚胎前胰岛对高浓度葡萄糖刺激无反应。但对高浓度葡萄糖加氨茶碱刺激有反应。在培养期间,猪胚胎前胰岛的 DNA 与腹岛素含量的比值不断增加。用过氧化物酶染色方法进行组织学检查,发现除少许胰岛素和高血糖素阳性的细胞外,在猪胚胎的胰腺组织和前胰岛内并没有成熟的胰岛。将猪胚胎前胰岛移植给于8只裸鼠的肾包膜下,于3～4周后静
In recent years, research on pig embryo anterior pancreatic islet transplantation has attracted more and more attention due to the lack of sources of adult and fetal pancreatic donor tissues and the inherent advantages of porcine prefetal islets. The second trimester gestational age of 75 ± 3 days of the embryo tissue of the pancreas, collagenase digestion mild, cultured for 10 days. The cultured proembryo islets do not respond to high concentrations of glucose. However, high concentrations of glucose plus aminophylline stimulation response. During culture, the ratio of DNA to pro-insulin content in porcine anterior fetal islets continues to increase. Histological examination with peroxidase staining revealed that there was no mature islet in the pancreatic tissue and anterior pancreatic islets in pig embryos except for a few insulin and glucagon positive cells. Pig porcine anterior pancreatic islets were implanted under the renal capsule of 8 nude mice and were calm after 3 to 4 weeks