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目的构建留置导尿管表面大肠杆菌生物膜(biofilm,BF)体内模型,研究巯乙磺酸钠对体内留置导尿管表面大肠杆菌BF的作用。方法家兔行导尿术,经导尿管注入大肠杆菌4 d,扫描电镜及平板计数法检测留置导尿管表面大肠杆菌BF动物模型构建;经导尿管灌注巯乙磺酸钠,扫描电镜观察巯乙磺酸钠对导尿管表面大肠杆菌BF的作用,平板计数法检测巯乙磺酸钠对导尿管表面细菌数的影响。结果模型组可见大量细菌在导尿管上呈团状或膜状黏附生长,厚薄不均的黏液状物质连接成一大片,平均菌落计数模型组(4.76±0.29)较对照组(2.49±0.22)明显增多(t=17.44,P<0.01);巯乙磺酸钠干预后,巯乙磺酸钠能减少留置导尿管表面大肠杆菌BF中基质样物质,仅见散在的细菌黏附于管壁上,有少数细菌的散在团状聚集;平均菌落计数与空白对照组(5.77±0.26)及生理盐水对照组(5.54±0.52)比较,巯乙磺酸钠组(2.85±0.36)能使BF中的细菌数明显减少(F=136.44,P<0.01)。结论留置导尿管表面大肠杆菌BF动物模型成功建立;巯乙磺酸钠对体内留置导尿管表面大肠杆菌BF有破坏作用。
Objective To construct an in vivo model of biofilm (BF) on the surface of indwelling catheter to study the effect of sodium thio-ethane sulfonate on the surface of colon urinary catheter E. coli BF. Methods The urethral catheterization was performed in rabbits. Escherichia coli was injected into the urethra for 4 days. The animal model of Escherichia coli BF was established by scanning electron microscopy (SEM) and plate count method. The catheter was infused with sodium thioethanesulfonate and scanning electron microscopy To observe the effect of sodium thio-ethane sulfonate on the surface of ureteral catheter Escherichia coli BF, and to detect the effect of sodium thio-ethane sulfonate on the number of bacteria on the surface of urinary catheter by plate count method. Results In the model group, a large number of bacteria adhered in a clumpy or membranous manner on the urinary catheter. The mucus with uneven thickness was connected into a large piece. The mean colony counting model group (4.76 ± 0.29) was significantly higher than that of the control group (2.49 ± 0.22) (T = 17.44, P <0.01). After sodium thioethane sulfonate intervention, sodium thio-ethane sulfonate could reduce the matrix-like substances in the colonies of E.coli BF on the catheter surface, only scattered bacteria adhered to the wall of the tube, The number of bacteria in the cluster was aggregated in a small number of bacteria. The average colony count was 2.85 ± 0.36 in the control group (5.77 ± 0.26) and saline control group (5.54 ± 0.52) Significantly reduced (F = 136.44, P <0.01). Conclusion The animal model of Escherichia coli BF was successfully established on the surface of indwelling catheter. Sodium thioethanesulfonate could destroy the surface of colon urinary catheter Escherichia coli BF.