Polarographic immunoassay coupled with catalysis of non-radioactive multiple iodine label

• 作 者: 宋俊峰 白亚丽 过玮 贾晓琳
• 机 构: Institute of Electroanalytical Chemistry,Northwest University,Xi an 710069,China,Institute of Electr
• 出 处: Science in China(Series B)
• 发表日期: 2004年期
• 关键词: catalysis iodine immunoassay covalently conjugate radioactive label heterogeneou

A now polarographic immunoassay was developed In this assay,human serum albumin (HSA) as the model antigen was covalently labeled with organic compound erythrosin B(EB) containing four non-radioactive iodides through Ⅰ step chemical reaction The labeling procedure is simple and the conditions needed are moderate.The molar labeling ratio of KB HSA was 12 Ⅰ The content of iodine in the conjugate obtained by the proposed procedure is ninth higher than that by the other existing methods.A heterogeneous competitive immunoassay was established by compling the catalysis of the conjugate to substrate As(Ⅲ)-Ce(Ⅳ) reaction with the linear-sweep polarographic detec-tion of As(Ⅲ) amount HSA can be determined in the HSA concentration range from 1 to 200μg/mL,with the de-tection hum of 0 66μg/ml.