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目的:对住院患者手术或输血前配血标本,均进行常规检测梅毒螺旋体抗体,以避免医院内感染及其引起的医疗纠纷。方法:采用酶联免疫吸附试验(ELISA),对2001年1月~2007年12月期间在本院住院的患者配血标本进行梅毒抗体初检,对初检阳性标本再用梅毒螺旋体颗粒凝聚试验(TPPA)法确认。结果:10498份输血前标本,用ELISA法检出梅毒抗体阳性56份,阳性率为0.53%。ELISA法检出的56份阳性标本,经TPPA法确认阳性52例,阳性检出率为0.48%。结论:ELISA法作为住院患者手术及输血前梅毒抗体常规方法,可以及时了解掌握医院内输血感染情况,确保医患安全。
OBJECTIVE: To routinely test syphilis antibodies against inpatients with preoperative blood transfusion or blood transfusion, to prevent nosocomial infections and medical disputes caused by them. Methods: The first phase of syphilis antibody test was performed on the blood samples of patients hospitalized in our hospital from January 2001 to December 2007 by enzyme-linked immunosorbent assay (ELISA) (TPPA) method to confirm. Results: Of the 10498 pretransfusion samples, 56 were positive for syphilis antibody by ELISA, the positive rate was 0.53%. Fifty-six positive samples were detected by ELISA, and 52 were confirmed by TPPA. The positive rate was 0.48%. Conclusion: As a routine method of inpatient operation and syphilis antibody before transfusion, ELISA can keep abreast of blood transfusion infection in hospitals and ensure the safety of doctors and patients.