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采用连续２次ＦｉｃｏｌＨｙｐａｑｕｅ分离，初步纯化脐血造血细胞，将其单个核细胞接种于经γ射线预处理的成人骨髓基质细胞层上，加入适量ＩＬ－３，并设不加ＩＬ－３的对照组，持续培养６周，定期检测非粘附细胞和集落形成细胞（ＣＦＣ）。结果：ＩＬ－３组３～６周非粘附细胞数和ＣＦＣ产率显著高于对照组，分别为对照组的２．７～４．５倍和１．６～２．３倍。说明ＩＬ－３在基质细胞支持的培养体系中可以促进脐血原始造血细胞的增殖分化
Umbilical cord blood hematopoietic cells were preliminarily purified by two consecutive FicolHypaque separations. Mononuclear cells were seeded on γ-ray-pretreated adult bone marrow stromal cells, and appropriate amount of IL-3 was added. The control group was cultured for 6 weeks, and non-adherent cells and colony forming cells (CFCs) were detected regularly. Results: The numbers of non-adherent cells and CFCs in IL-3 group were significantly higher than those in control group at 3 to 6 weeks, which were 2.7 to 4.5 times and 1.6 to 2.3 times that of control group respectively. IL-3 in stromal cell-supported culture system can promote the proliferation and differentiation of cord blood hematopoietic progenitor cells