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本文叙述了用水蒸气蒸馏程序将鱼贝类中甲基汞抽出,然后用气相色谱法定量。操作步骤如下:称取切碎混匀的样品5～50g(称准至0.001g)置于500ml蒸馏烧瓶内,加入6N盐酸40ml和氯化钠40g,通入蒸气进行蒸馏。馏出液吸收于10ml 3 N盐酸中,使蒸至180ml为止,然后用水加至200ml。移取馏出液50ml于100ml分液漏斗中,分别用30ml和20ml的苯抽取2次。合并苯层,用20%氯化钠溶液20ml洗涤一次后,在苯层中加入6ml半胱氨酸醋酸溶液,甲基汞便转溶于半胱氨酸醋酸溶液层。然后移取4ml置于10ml具塞试管内,加入2ml 6 N盐酸溶液和4ml内标准液,甲基汞便被移至内标准液中。将内
This paper describes the extraction of methylmercury from fish and shellfish using a steam distillation procedure and then quantification by gas chromatography. Operation steps are as follows: Weigh chopped and mixed sample 5 ~ 50g (weighed to 0.001g) placed in 500ml distillation flask, adding 6N hydrochloric acid 40ml and sodium chloride 40g, steaming distillation. The distillate was taken up in 10 ml of 3 N hydrochloric acid, steamed to 180 ml and then added to 200 ml with water. Pipette distillate 50ml in 100ml separatory funnel, respectively, with 30ml and 20ml of benzene extracted twice. Combine the benzene layers and wash once with 20 ml of 20% sodium chloride solution. Then 6 ml of cysteine acetic acid solution is added to the benzene layer, and methylmercury is dissolved in the cysteine acetic acid solution layer. Then remove 4ml placed in 10ml stopper tube, add 2ml 6N hydrochloric acid solution and 4ml internal standard solution, methylmercury will be moved to the internal standard solution. Will be inside